Reference : Kinetics of interaction between the exocellular DD-carboxypeptidase-transpeptidase fr...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Life sciences : Microbiology
http://hdl.handle.net/2268/83081
Kinetics of interaction between the exocellular DD-carboxypeptidase-transpeptidase from Streptomyces R61 and beta-lactam antibiotics. A choice of models
English
Frère, Jean-Marie mailto [Université de Liège - ULg > Faculté de Médecine, Institut de Botanique > Service de Microbiologie > >]
Ghuysen, Jean-Marie [Université de Liège - ULg > Faculté de Médecine, Institut de Botanique > Service de Microbiologie > > >]
Iwatsubo, Motohiro [Centre National de la Recherche Scientifique - CNRS > Centre de Génetique Moléculaire > > >]
15-Sep-1975
European Journal of Biochemistry
Blackwell Science
57
2
343-351
Yes (verified by ORBi)
International
0014-2956
1432-1033
Oxford
United Kingdom
[en] acyltransferases/*metabolism ; ampicillin/metabolism ; binding sites ; carbenicillin/metabolism ; carboxypeptidases/*metabolism ; kinetics ; mathematics ; penicillin g/metabolism ; penicillins/*metabolism ; peptidyl transferases/*metabolism ; protein binding ; streptomyces/*enzymology
[en] The simplest model for the interaction between the exocellular DD-carboxypeptidase-transpeptidase from Streptomyces R61 and beta-lactam antibiotics involves the three following steps: (a) the formation of a reversible equimolar enzyme - antibiotic complex; (b) the irreversible transformation of this complex into a modified enzyme - antibiotic complex; and (c) the breakdown of this latter complex and the concomitant release of a regenerated enzyme and a modified antibiotic molecule. The dissociation constant for step 1 and the rate constants for steps 2 and 3 were measured with various beta-lactam antibiotics. With antibiotic such as benzylpenicillin, which behaves as a good 'substrate', steps 1 and 2 occur at enzymic velocities, whereas step 3 occurs at a very low velocity and hence is responsible for the low efficiency of the overall process.
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Fonds de la Recherche Fondamentale Collective
Researchers ; Professionals
http://hdl.handle.net/2268/83081

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