Article (Scientific journals)
Interactions between beta-lactam antibiotics and isolated membranes of Streptococcus faecalis ATCC 9790
Coyette, Jacques; Ghuysen, Jean-Marie; Binot, Françoise et al.
1977In European Journal of Biochemistry, 75 (1), p. 231-239
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Keywords :
binding sites; carboxypeptidases/*metabolism; cell membrane/*enzymology; enterococcus faecalis/*enzymology; kinetics; muramoylpentapeptide carboxypeptidase/*metabolism; penicillin g/analogs & derivatives/*metabolism; penicillins/*metabolism; protein binding; structure-activity relationship
Abstract :
[en] The DD-carboxypeptidase-exchange membrane-bound enzyme in Streptococcus faecalis ATCC 9790 reacts with beta-lactam antibiotics to form complexes with rather long half-lives. Depending upon the antibiotic, the second-order rate constants for complex formation range from 0.75-560 M-1 S-1 (at 37 degrees C and in water) and the first-order rate constants for complex breakdown range from 1.3 to 26 x 10(-5) s-1 (at 37 degrees C and in 5 mM phosphate buffer pH 7.5). There are about 30 pmol of DD-carboxypeptidase-exchange enzyme per mg of membrane protein. The degradation products arising from benzylpenicillin are phenylacetylglycine and probably N-formyl-D-penicillamine. Isolated membranes also contain other penicillin binding sites (about 70 pmol/mg membrane protein). That part of benzylpenicillin which reacts with at least some of these latter sites is slowly degraded into penicilloic acid. Normal functioning of the DD-carboxypeptidase-exchange membrane-bound enzyme is important, if not essential, for cell growth. With the beta-lactam antibiotics tested inhibition of cell growth is mainly related to the rates of formation of the inactive enzyme-antibiotic complexes. The relationship, however, is not a direct one probably due to the competitive effect exerted by the other penicillin binding sites.
Disciplines :
Biochemistry, biophysics & molecular biology
Microbiology
Author, co-author :
Coyette, Jacques;  Université de Liège - ULiège > Faculté de Médecine > Service de Microbiologie
Ghuysen, Jean-Marie ;  Université de Liège - ULiège > Faculté de Médecine > Service de Microbiologie
Binot, Françoise ;  Université de Liège - ULiège > Faculté de Médecine > Service de Microbiologie
Adriaens, Paul;  Katholieke Universiteit Leuven - KUL > Rega Instituut
Meesschaert, Boudewijn;  Katholieke Universiteit Leuven - KUL > Rega Instituut
Vanderhaeghe, Hubert;  Katholieke Universiteit Leuven - KUL > Rega Instituut
Language :
English
Title :
Interactions between beta-lactam antibiotics and isolated membranes of Streptococcus faecalis ATCC 9790
Publication date :
02 May 1977
Journal title :
European Journal of Biochemistry
ISSN :
0014-2956
eISSN :
1432-1033
Publisher :
Blackwell Science, Oxford, United Kingdom
Volume :
75
Issue :
1
Pages :
231-239
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
NIH - National Institutes of Health [US-MD]
FRFC - Fonds de la Recherche Fondamentale Collective [BE]
Available on ORBi :
since 28 January 2011

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