Article (Scientific journals)
Interaction between penicillin and the DD-carboxypeptidase of the unstable L-form of Proteus mirabilis strain 19
Schilf, Wolfgang; Frere, Philippe; Frère, Jean-Marie et al.
1978In European Journal of Biochemistry, 85 (2), p. 325-330
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Keywords :
carboxypeptidases/metabolism; kinetics; mathematics; penicillins; protein binding; proteus mirabilis/*enzymology; spheroplasts/enzymology
Abstract :
[en] Binding of penicillin to the DD-carboxypeptidase of the unstable spheroplast L-form of Proteus mirabilis results in the rapid formation of a modified enzyme-inhibitor complex which in turn undergoes rapid decay into reactivated enzyme and an antibiotically inactive penicillin degradation product. Major antibiotic metabolites recovered from such interactions were benzylpenicilloic acid and phenoxymethylpenicilloic acid from benzylpenicillin and phenoxymethylpenicillin, respectively, suggesting a second enzymic function of the DD-carboxypeptidase as a penicillinase of low efficiency. Statistical analyses made with the help of a linear regression program show that the enzyme interacts with the substrate UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-(L)-meso-2,6-diaminopimelyl -(L)-D-alanyl-D-alanine and either benzympenicillin or carbenicillin in a non-competitive manner.
Disciplines :
Biochemistry, biophysics & molecular biology
Microbiology
Author, co-author :
Schilf, Wolfgang;  Technische Hochschule (Darmstadt) > Institut fur Mikrobiologie, Fachbereich Biologie
Frere, Philippe;  Ateliers des Constructions Electriques de Charleroi > Centre de Calcul
Frère, Jean-Marie ;  Université de Liège - ULiège > Faculté de Médecine > Service de Microbiologie
Martin, Hans-Herbert;  Technische Hochschule Darmstadt > Institut fur Mikrobiologie, Fachbereich Biologie
Ghuysen, Jean-Marie ;  Université de Liège - ULiège > Faculté de Médecine > Service de Microbiologie
Adriaens, Paul;  Université Catholique de Louvain - UCL > Rega Instituut > Afdeling Microbiologie
Meesschaert, Boudewijn;  Université Catholique de Louvain - UCL > Rega Instituut > Afdeling Microbiologie
Language :
English
Title :
Interaction between penicillin and the DD-carboxypeptidase of the unstable L-form of Proteus mirabilis strain 19
Publication date :
17 April 1978
Journal title :
European Journal of Biochemistry
ISSN :
0014-2956
eISSN :
1432-1033
Publisher :
Blackwell Science, Oxford, United Kingdom
Volume :
85
Issue :
2
Pages :
325-330
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
NIH - National Institutes of Health [US-MD]
Available on ORBi :
since 27 January 2011

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