Article (Scientific journals)
Isolation of the membrane-bound 26 000-Mr penicillin-binding protein of Streptomyces strain K15 in the form of a penicillin-sensitive D-alanyl-D-alanine-cleaving transpeptidase
Nguyen-Distèche, Martine; Leyh-Bouille, Mélina; Ghuysen, Jean-Marie
1982In Biochemical Journal, 207 (1), p. 109-115
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Keywords :
bacterial proteins; carboxypeptidases/*isolation & purification; carrier proteins/*isolation & purification/metabolism; cell membrane/enzymology; cetrimonium compounds; chromatography, affinity; chromatography, gel; detergents; electrophoresis, polyacrylamide gel; hexosyltransferases; muramoylpentapeptide carboxypeptidase/antagonists & inhibitors/*isolation; purification/metabolism; penicillin g/*pharmacology; penicillin-binding proteins; peptidyl transferases; streptomyces/*enzymology
Abstract :
[en] The membrane-bound, 26 000-Mr penicillin-binding protein of Streptomyces K15 has been isolated in the form of an effective, penicillin-sensitive D-alanyl-D-alanine-cleaving peptidase exhibiting high transpeptidase activity (greater than 95%) and very low carboxy-peptidase activity (less than 5%). The penicillin-binding protein/transpeptidase can be extracted directly from the mycelium with N-cetyl-NNN-trimethylammonium bromide (Cetavlon) and subsequently obtained at 90% purity and with an 8000-fold specific enrichment (when compared with the activity of the isolated membranes) by a two-step procedure involving Sephadex filtration and affinity chromatography on ampicillin-linked CH Sepharose 4B in the presence of detergent. At saturating concentrations of the co-substrates diacetyl-L-Lys-D-Ala-D-Ala and Gly-Gly, the catalytic-centre activity is about 0.3 s-1.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Nguyen-Distèche, Martine ;  Université de Liège - ULiège > Faculté de Médecine, Institut de Chimie > Service de Microbiologie
Leyh-Bouille, Mélina;  Université de Liège - ULiège > Faculté de Médecine, Institut de Chimie > Service de Microbiologie
Ghuysen, Jean-Marie ;  Université de Liège - ULiège > Faculté de Médecine, Institut de Chimie > Service de Microbiologie
Language :
English
Title :
Isolation of the membrane-bound 26 000-Mr penicillin-binding protein of Streptomyces strain K15 in the form of a penicillin-sensitive D-alanyl-D-alanine-cleaving transpeptidase
Publication date :
01 October 1982
Journal title :
Biochemical Journal
ISSN :
0264-6021
eISSN :
1470-8728
Publisher :
Portland Press, London, United Kingdom
Volume :
207
Issue :
1
Pages :
109-115
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FRSM - Fonds de la Recherche Scientifique Médicale [BE]
NIH - National Institutes of Health [US-MD]
Available on ORBi :
since 27 January 2011

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