Typing of Nosocomial Strains of Serratia Marcescens: Comparison of Pulsed-Field Gel Electrophoresis of Macrorestriction Fragments with Biotyping, Esterase Typing and Ribotyping
[en] Fifty nosocomial isolates of Serratia marcescens, collected in six Belgian hospitals between 1986 and 1990, were characterized by using pulsed-field gel electrophoresis (PFGE) with XbaI. The results were compared with those previously obtained by three other methods: biotyping, esterase electrophoresis typing and ribotyping with EcoRI and HindIII. Macrorestriction analysis (42 PFGE groups) and esterase typing (42 zymotypes) proved to be the most discriminating, followed by ribotyping (28 ribotypes) and biotyping (10 biochemical profiles). Biotyping would serve as a screen to identify isolates, due to its accessibility. Esterase typing provided a reliable tool to make subdivisions within biotypes because of congruence between biochemical groups and esterase patterns. Additional discrimination was still achieved by ribotyping and PFGE. It is concluded that the combined results of these four markers were useful for distinguishing all epidemic and sporadic isolates.
Disciplines :
Microbiology
Author, co-author :
Chetoui, Hafid; Université de Liège - ULiège > microbiologie médicale
Delhalle, Edmond; Université de Liège - ULiège > microbiologie médicale
Melin, Pierrette ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Microbiologie médicale et virologie médicale
Struelens, Marc J; Cliniques Universitaires de Bruxelles - Hôpital Erasme > Microbiologie médicale
De Ryck, R.
Osterrieth, Paul; Université de Liège - ULiège > Microbiologie médicale
De Mol, Patrick ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Microbiologie médicale et virologie médicale
Language :
English
Title :
Typing of Nosocomial Strains of Serratia Marcescens: Comparison of Pulsed-Field Gel Electrophoresis of Macrorestriction Fragments with Biotyping, Esterase Typing and Ribotyping
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