Reference : Typing of Nosocomial Strains of Serratia Marcescens: Comparison of Pulsed-Field Gel Elec...

	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Microbiology
	To cite this reference:	http://hdl.handle.net/2268/82454

Title :	Typing of Nosocomial Strains of Serratia Marcescens: Comparison of Pulsed-Field Gel Electrophoresis of Macrorestriction Fragments with Biotyping, Esterase Typing and Ribotyping
Language :	English
Author, co-author :	Chetoui, Hafid [Université de Liège - ULg > > microbiologie médicale > >]
	Delhalle, Edmond [Université de Liège - ULg > > > > microbiologie médicale > >]
	Melin, Pierrette [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Microbiologie médicale et virologie médicale]
	Struelens, Marc J [Cliniques Universitaires de Bruxelles - Hôpital Erasme > > > > Microbiologie médicale > >]
	De Ryck, R. [> > > >]
	Osterrieth, Paul [Université de Liège - ULg > > > > Microbiologie médicale > >]
	De Mol, Patrick [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Microbiologie médicale et virologie médicale]
Publication date :	Feb-1998
Journal title :	Research in Microbiology
Volume :	149
Issue/season :	2
Pages :	137-43
Audience :	International
ISSN :	0923-2508
Keywords :	[en] Serratia ; nosocomial ; typing ; PFGE ; ribotyping
Abstract :	[en] Fifty nosocomial isolates of Serratia marcescens, collected in six Belgian hospitals between 1986 and 1990, were characterized by using pulsed-field gel electrophoresis (PFGE) with XbaI. The results were compared with those previously obtained by three other methods: biotyping, esterase electrophoresis typing and ribotyping with EcoRI and HindIII. Macrorestriction analysis (42 PFGE groups) and esterase typing (42 zymotypes) proved to be the most discriminating, followed by ribotyping (28 ribotypes) and biotyping (10 biochemical profiles). Biotyping would serve as a screen to identify isolates, due to its accessibility. Esterase typing provided a reliable tool to make subdivisions within biotypes because of congruence between biochemical groups and esterase patterns. Additional discrimination was still achieved by ribotyping and PFGE. It is concluded that the combined results of these four markers were useful for distinguishing all epidemic and sporadic isolates.
Permalink :	http://hdl.handle.net/2268/82454

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