ORBi: Lejeune Jean-Philippe - Effect of intensive exercise on plasmatic neutrophil elastase level in eventing and endurance horses

Reference : Effect of intensive exercise on plasmatic neutrophil elastase level in eventing and endu...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Veterinary medicine & animal health
	To cite this reference:	http://hdl.handle.net/2268/81636

Title :	Effect of intensive exercise on plasmatic neutrophil elastase level in eventing and endurance horses
Language :	English
Author, co-author :	Lejeune, Jean-Philippe [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
	Sandersen, Charlotte [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie et réanimation vétérinaires >]
	Votion, Dominique [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
	Caudron, Isabelle [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Département clinique des animaux de compagnie et des équidés >]
	Vander Heyden, Laurent [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
	Franck, Thierry [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
	Ceusters, Justine [Université de Liège - ULg > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
	Mouithys-Mickalad, Ange [Université de Liège - ULg > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
	Niesten, Ariane [Université de Liège - ULg > > Centre de l'oxygène : Recherche et développement (C.O.R.D.) >]
	de la Rebière de Pouyade, Geoffroy [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
	Serteyn, Didier [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux >]
Publication date :	2010
Journal title :	Equine Veterinary Journal. Supplement
Publisher :	Equine Veterinary Journal Ltd
Volume :	48
Pages :	12-16
Audience :	International
ISSN :	0425-1644
City :	Newmarket Suffolk
Country :	United Kingdom
Keywords :	[en] horse ; equine physiology ; intensive exercise ; elastase ; myeloperoxidase ; inflammation
Abstract :	[en] Reasons for performing the study – Intensive exercise induces a systemic inflammatory response characterized by an increase of blood neutrophil count and myeloperoxidase (MPO) release. Neutrophil elastase (NE) could also contribute to tissues lesions by their proteinase activities. Objective – To compare plasmatic NE concentrations before and after different forms of intensive exercise. Materials and Methods – EDTA blood samples were taken from 51 eventing horses (EvH) and 32 endurance horses (EndH) were sampled before the race (T0). Blood sampling was performed 2 h (T1) after completing either phase D of a one or two star eventing competition (n=51) or a 120 or 160 km endurance race (n=32). Plasmatic NE and MPO were measured by a specific equine ELISA. Neutrophil counts and creatine kinase (CK) levels were also measured. A Wilcoxon test for paired samples was used to compare mean values of neutrophils, CK, MPO and NE at T0 and T1 in EvH and in EndH. Correlations were calculated between all the 4 parameters in EvH and EndH. Results – At T0, mean NE levels were 14.43 ± 3.63 ng/ml for EvH and 11.7 ± 2.11 ng/ml for EndH. The competition induced a significant increase of NE levels in (58.57 ± 24.06 ng/mL) EvH and (95.74 ± 22.70 ng/mL) EndH (p < 0,05). NE was significantly (p < 0,0001) correlated to MPO in EvH (r = 0.293) and EndH (0.594) and to CK (r = 0.297) in EndH (p<0.0001). Neutrophils, CK and MPO were significantly increased between T0 and T1 in both types of horses. Conclusions – Significant increase of NE was observed after intense exercise with a significant correlation between NE and MPO. The huge variability in MPO and ELT, indicates, that not all horses show the same intensity of systemic inflammatory response.
Funders :	Région wallonne : Ministère de l'agricuture et de la ruralité
Target :	Researchers ; Professionals ; Students
Permalink :	http://hdl.handle.net/2268/81636

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