Reference : The Importance of the Negative Charge of Beta-Lactam Compounds for the Inactivation of t...
Scientific journals : Article
Life sciences : Microbiology
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/81287
The Importance of the Negative Charge of Beta-Lactam Compounds for the Inactivation of the Active-Site Serine Dd-Peptidase of Streptomyces R61
English
Varetto, Louis mailto [Université de Liège - ULg > Institut de Chimie > Service de Microbiologie > >]
Frère, Jean-Marie mailto [Université de Liège - ULg > Institut de Chimie > Service de Microbiologie > >]
Ghuysen, Jean-Marie [Université de Liège - ULg > Institut de Chimie > Service de Microbiologie > > >]
10-Dec-1987
FEBS Letters
Elsevier Science
225
1-2
218-222
Yes (verified by ORBi)
International
0014-5793
Amsterdam
The Netherlands
[en] beta-lactam ; pH dependence ; enzyme inactivation ; deacetyl-cephalosporin c ; dd-peptidase
[en] The interaction between the Streptomyces R61 penicillin-sensitive DD-peptidase and deacetyl-cephalosporin C or its lactone derivative has been studied at different pH values. The results show the importance of an enzyme group of pK approximately equal to 9 which might form an ion pair with the free carboxylate of the former compound. This electrostatic interaction is shown to contribute to the formation of the first, non-covalent enzyme-inactivator complex by a factor of at least 50.
Fonds de la Recherche Scientifique Médicale - FRSM
Researchers ; Professionals
http://hdl.handle.net/2268/81287
10.1016/0014-5793(87)81161-4

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