Article (Scientific journals)
Active-Site and Membrane Topology of the Dd-Peptidase/Penicillin-Binding Protein No. 6 of Enterococcus Hirae (Streptococcus Faecium) A.T.C.C. 9790
El Kharroubi, Aboubaker; Piras, Graziella; Jacques, Philippe et al.
1989In Biochemical Journal, 262 (2), p. 457-462
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Keywords :
Topology; C terminal-Sequence; Homology; Isoelectrical focusing; Proteins; Enzyme; Binding protein; Antibiotic; Active site; Binding site; Molecular structure; Chromatography; Microorganism culture; Gel electrophoresis; Radiolabelling; Biomembrane; Topologie; Séquence C terminale; Homologie; Focalisation isoélectrique; Culture microorganisme; Chromatographie; Membrane biologique; Marquage radioisotopique; Electrophorèse gel; Structure moléculaire; Site fixation; Site actif; Antibiotique; Protéine de liaison; Protéine; Topología; Membrana biológica; Marcación radioisotópica; Electroforesis gel; Cultivo microorganismo; Cromatografía; Estructura molecular; Sitio fijación; Lugar activo; Antibiótico; Proteina de enlace; Enzima; Proteina; Focalización isoeléctrica; Homología; Secuencia C terminal
Abstract :
[en] The membrane-bound 43,000-Mr penicillin-binding protein no. 6 (PBP6) of Enterococcus hirae consists of a 30,000-Mr DD-peptidase/penicillin-binding domain and a approximately 130-residue C-terminal appendage. Removal of this appendage by trypsin proteolysis has no marked effect on the catalytic activity and penicillin-binding capacity of the PBP. Anchorage of the PBP in the membrane appears to be mediated by a short 15-20-residue stretch at the C-terminal end of the appendage. The sequence of the 50-residue N-terminal region of the PBP shows high degree of homology with the sequences of the corresponding regions of the PBPs5 of Escherichia coli and Bacillus subtilis. On this basis the active-site serine residue occurs at position 35 in the enterococcal PBP.
Disciplines :
Biochemistry, biophysics & molecular biology
Microbiology
Author, co-author :
El Kharroubi, Aboubaker;  Université de Liège - ULiège > Institut de Chimie > Service de Microbiologie
Piras, Graziella;  Université de Liège - ULiège > Institut de Chimie > Service de Mircobiologie
Jacques, Philippe ;  Université de Liège - ULiège > Institut de Chimie > Service de Microbiologie
Szabo, Istvan;  Rijksuniversiteit-Gent > Laboratorium voor Microbiologie
Van Beeumen, Jozef;  Rijksuniversiteit-Gent > Laboratorium voor Microbiologie
Coyette, Jacques ;  Université de Liège - ULiège > Institut de Chimie > Service de Microbiologie
Ghuysen, Jean-Marie ;  Université de Liège - ULiège > Institut > Service de Microbiologie
Language :
English
Title :
Active-Site and Membrane Topology of the Dd-Peptidase/Penicillin-Binding Protein No. 6 of Enterococcus Hirae (Streptococcus Faecium) A.T.C.C. 9790
Publication date :
01 September 1989
Journal title :
Biochemical Journal
ISSN :
0264-6021
eISSN :
1470-8728
Publisher :
Portland Press, London, United Kingdom
Volume :
262
Issue :
2
Pages :
457-462
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FRSM - Fonds de la Recherche Scientifique Médicale [BE]
Available on ORBi :
since 07 January 2011

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