Reference : Prolactin-induced activation of nuclear factor kappa B in bovine mammary epithelial c...
Scientific journals : Article
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/8053
Prolactin-induced activation of nuclear factor kappa B in bovine mammary epithelial cells: Role in chronic mastitis
English
Boutet, Philippe mailto [Université de Liège - ULg > Département de sciences fonctionnelles > GIGA-R : Biochimie et biologie moléculaire >]
Sulon, Joseph [Université de Liège - ULg > Département de sciences fonctionnelles > Physiologie de la reproduction >]
Closset, R. [ > > ]
Detilleux, J. [ > > ]
Beckers, Jean-François mailto [Université de Liège - ULg > Département de sciences fonctionnelles > Physiologie de la reproduction >]
Bureau, Fabrice mailto [Université de Liège - ULg > Département de sciences fonctionnelles > Biochimie et biologie moléculaire >]
Lekeux, Pierre mailto [Université de Liège - ULg > Département de sciences fonctionnelles > Physiologie >]
2007
Journal of Dairy Science
Amer Dairy Science Assoc
90
1
155-164
Yes (verified by ORBi)
International
0022-0302
1525-3198
Savoy
[en] bovine mastitis ; prolactin ; nuclear factor kappa B ; cytokine
[en] We sought to determine whether prolactin (PRL) could influence the neutrophilic inflammation that characterizes chronic mastitis. Most of the genes encoding inflammatory proteins depend on the nuclear factor kappa B (NF-kappa B) for their expression. We addressed the hypothesis that immunomodulatory activities of PRL might arise from an increase in NF-kappa B activity. MAC-T cells, a bovine mammary epithelial cell line, were stimulated with increasing concentrations of bovine PRL ( 1, 5, 25, 125, and 1,000 ng/mL). Level of NF-kappa B binding activity was measured and mRNA was evaluated for IL-1 beta, IL-6, IL-8, granulocyte-macrophage colony-stimulating factor (GMCSF), IFN-gamma, and tumor necrosis factor (TNF)-alpha, cytokines known to require NF-kappa B for their maximal transcription. Prolactin activated NF-kappa B; maximal NF-kappa B activation was weaker with PRL than with TNF-alpha at 30 or 180 min poststimulation. In addition, PRL significantly amplified, in a dose-dependent manner, mRNA expression of IL-1 beta, IL-6, IL-8, GMCSF, and TNF-a. We measured PRL concentrations in blood and milk from healthy and chronic mastitis-infected cows, and studied the relationship between the PRL concentration and the degree of inflammation in the mammary gland as indirectly assessed by somatic cell counts (SCC). Plasma PRL did not differ significantly between healthy and chronic mastitis-affected cows (63.7 and 67.5 ng/mL, respectively). Milk PRL concentration was significantly increased in chronic mastitis-affected quarters with the highest SCC, and had a positive significant correlation between SCC, as well as between the number of neutrophils present in milk samples. The present findings show that PRL promotes an inflammatory response in bovine mammary epithelial cells via NF-kappa B activation, and suggest a role for PRL in the pathogenesis of chronic mastitis.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/8053

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