Reference : Presence of human antibodies reacting with Candida albicans O-linked oligomannosides ...
Scientific journals : Article
Human health sciences : Laboratory medicine & medical technology
http://hdl.handle.net/2268/80494
Presence of human antibodies reacting with Candida albicans O-linked oligomannosides revealed by using an enzyme-linked immunosorbent assay and neoglycolipids
English
Hayette, Marie-Pierre mailto [Université de Liège - ULg > > Microbiologie médicale >]
Strecker, Gerald [ > > ]
Faille, Christine [ > > ]
Dive, Daniel [ > > ]
Camus, Daniel [ > > ]
Mackenzie, D. W. [ > > ]
Poulain, Daniel mailto [ > > ]
Feb-1992
Journal of Clinical Microbiology
American Society for Microbiology (ASM)
30
2
411-417
Yes (verified by ORBi)
International
0095-1137
1098-660X
Washington
DC
[en] Candida albicans ; neoglycolipids ; ELISA
[en] In order to study the presence of antibodies directed against Candida albicans O-linked oligomannosides (oligomannosides O) in patient sera, we have developed an enzyme-linked immunosorbent assay (ELISA) involving neoglycolipids constructed with these residues (NGLO). Oligomannosides O released by mild alkaline degradation of the C. albicans cell wall phosphopeptidomannan (PPM) contained one to seven mannose residues, among which the quantitatively major components, mannobiose and mannotriose, were shown by 1H nuclear magnetic resonance to contain exclusively alpha (1-2) linkages. The pool of oligomannosides was converted to neoglycolipids by coupling them to 4-hexadecylaniline in an equimolar reaction checked by thin-layer chromatography. We have tested against these neoantigens, coated on ELISA plates, 15 pairs of sera corresponding to individual seroconversions observed in 15 patients during the course of a mycological and serological survey of candidiasis. For all patients, seroconversions resulted in an increased level of antibodies against NGLO. A significant correlation was observed between the results of ELISA-NGLO, ELISA involving the original PPM molecule, and routine antibody detection tests, indirect immunofluorescence assay, and cocounterimmunoelectrophoresis. These results therefore demonstrate the synthesis of human antibodies reactive with oligomannosides O constitutive of the C. albicans mannan molecule which have been previously described as exhibiting an inhibitory effect on human lymphocytic proliferation.
Researchers ; Professionals
http://hdl.handle.net/2268/80494

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