[en] Study of the Zn2+-containing D-alanyl-D-alanine-cleaving carboxypeptidase of Streptomyces albus G by small-angle X-ray scattering in solution yielded the following molecular parameters: radius of gyration R = 1.82 +/- 0.05 nm; largest diameter D = 5.9 +/- 0.2 nm; relative molecular mass Mr = 17000 +/- 2000; volume V approximately equal to 35 +/- 2 nm3; degree of hydration: 0.25 +/- 0.02 g water/g protein. By reference to theoretical scattering curves of rigid triaxial homogeneous bodies, a model which fits all experimental data is an elliptical cylinder. Such a model is compatible with that observed in the crystal structure. At those high concentrations necessary to form inactive enzyme-ligand associations the non-competitive beta-lactam inhibitors, cephalothin and cephalosporin C, drastically altered the scattering behaviour of the protein.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Labischinski, Harald; Robert-Koch-Institut des Bundesgesundheitsamtes
Giesbrecht, Peter; Robert-Koch-Institut des Bundesgesundheitsamtes
Fischer, E.; Institut Für Kristallographie der Freien Universität Berlin
Barnickel, G.; Institut Für Kristallographie der Freien Universität Berlin
Bradaczek, H.; Institut Für Kristallographie der Freien Universität Berlin
Frère, Jean-Marie ; Université de Liège - ULiège > Faculté de Médecine, Institut de Chimie Organique et de Biochimie > Laboratoire de Microbiologie
Houssier, Claude ; Université de Liège - ULiège > Institut de Chimie > Laboratoire de Chimie Physique
Charlier, Paulette ; Université de Liège - ULiège > Faculté de Médecine, Institut de Chimie Organique et de Biochimie > Laboratoire de Microbiologie
Dideberg, Otto; Université de Liège - ULiège > Institut de Physique > Laboratoire de Cristallographie
Ghuysen, Jean-Marie ; Université de Liège - ULiège > Faculté de Médecine, Institut de Chimie Organique et de Biochimie > Laboratoire de Microbiologie
Language :
English
Title :
Study of the Zn-containing DD-carboxypeptidase of Streptomyces albus G by small-angle X-ray scattering in solution.
Publication date :
1984
Journal title :
European Journal of Biochemistry
ISSN :
0014-2956
eISSN :
1432-1033
Publisher :
Blackwell Science, Oxford, United Kingdom
Volume :
138
Issue :
1
Pages :
83-87
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
FRSM - Fonds de la Recherche Scientifique Médicale [BE]
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