Reference : Cytokine production from sputum cells and blood leukocytes in asthmatics according to di...
Scientific journals : Article
Human health sciences : Cardiovascular & respiratory systems
http://hdl.handle.net/2268/79838
Cytokine production from sputum cells and blood leukocytes in asthmatics according to disease severity.
English
Manise, Maïté [Université de Liège - ULg > Département des sciences cliniques > Pneumologie - Allergologie >]
Schleich, FLorence mailto [Centre Hospitalier Universitaire de Liège - CHU > > Pneumologie-Allergologie >]
Gusbin, Natacha [Centre Hospitalier Universitaire de Liège - CHU > > Pneumologie-Allergologie >]
Godinas, Laurent mailto [Centre Hospitalier Universitaire de Liège - CHU > > Frais communs médecine >]
Henket, Monique mailto [Centre Hospitalier Universitaire de Liège - CHU > > Pneumologie-Allergologie >]
Antoine, Nadine [Centre Hospitalier Universitaire de Liège - CHU > > Bloc opératoire - ORL >]
Corhay, Jean-Louis mailto [Centre Hospitalier Universitaire de Liège - CHU > > Pneumologie-Allergologie >]
Louis, Renaud mailto [ULg > > > >]
2010
Allergy
Blackwell Publishing
65
7
889-96
Yes (verified by ORBi)
International
0105-4538
1398-9995
Oxford
United Kingdom
[en] BACKGROUND: Although mild to moderate asthma is known to be Th2 driven, cytokines produced in refractory asthma might not fit the classical Th2 pattern. METHODS: The aim of our study was to assess the cytokine production by sputum and blood cells from 15 refractory asthmatics (American Thoracic Society Criteria) compared to 15 mild untreated and 17 moderate treated asthmatics and 22 healthy subjects. Spontaneous production of interleukin (IL)-4, IL-6, IL-10, interferon-gamma, and tumor necrosis factor alpha was measured by immunotrapping after 24 h sputum or blood cell culture. RESULTS: Moderate and refractory asthmatics were both characterized by a lower production of IL-6 from their airway cells compared to healthy subjects. However, the difference was no longer significant when expressing the results per gram of sputum. No significant difference between the three groups was found regarding other cytokines. As for cytokine production from blood, the three groups of asthmatics exhibited raised production of IL-4 when compared to healthy subjects, and this was true when results were expressed per blood volume or after normalization for total leukocyte cell count. Moderate asthmatics exhibited greater production of IL-10 when compared to refractory asthmatics and healthy subjects when results were normalized for total leukocyte cell count. CONCLUSIONS: Sputum cells from moderate and refractory asthmatics release less IL-6. While the systemic overproduction of IL-4 was observed through the all spectrum of asthma severity, moderate asthmatics exhibited greater systemic IL-10 production compared to refractory asthmatics.
http://hdl.handle.net/2268/79838
10.1111/j.1398-9995.2009.02296.x
http://onlinelibrary.wiley.com/doi/10.1111/j.1398-9995.2009.02296.x/pdf

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