Article (Scientific journals)
Radioimmunoassay of bovine placental lactogen using recombinant and native preparations: determination of fetal concentrations across gestation
Alvarez-Oxiley, A. V.; Melo de Sousa, Noelita; Hornick, Jean-Luc et al.
2007In Reproduction, Fertility and Development, 19 (7), p. 877-885
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Keywords :
fetal serum; glycosylated form
Abstract :
[en] Concentrations of bovine placental lactogen ( bPL) were determined in fetal plasma samples by twelve double-antibody competitive radioimmunoassay systems ( RIA I - XII) based on either recombinant bPL ( non- glycosylated) or native bPL ( glycosylated). Both preparations were used as standard and tracer, and for primary antisera production. The minimum detection limit measured by these RIA varied from 0.02 to 0.6 ng bPLmL(-1). The coefficients of correlation of different bPL RIA systems were up to 90% ( P< 0.0001) when each RIA was tested against the average values of all twelve RIA systems. All developed RIA were used to investigate the incidence of different bPL isoforms in bovine fetal serum samples ( n= 71). Fetal concentrations ranged from 11.8 to 35.7 ng mL(-1) at the third month and from 1.1 to 13.5 ngmL(-1) at the ninth month of gestation. They tended to decrease with advancing gestation. In general, those RIA systems that used recombinant bPL as the standard measured higher values than those using the native bPL preparation. These differences decreased toward the end of gestation ( P< 0.05), suggesting a lower rate of glycosylation. Our results provide evidence of different glycosylated isoforms of bPL in fetal serum at different gestation periods.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Alvarez-Oxiley, A. V.
Melo de Sousa, Noelita ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Hornick, Jean-Luc  ;  Université de Liège - ULiège > Département de productions animales > Nutrition des animaux domestiques
Touati, Kamal ;  Université de Liège - ULiège > Département clinique des animaux de production (DCP) > Département clinique des animaux de production (DCP)
van der Weijden, G. C.
Taverne, M. A. M.
Szenci, O.
Sulon, Joseph ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Debliquy, P.
Beckers, Jean-François  ;  Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction
Language :
English
Title :
Radioimmunoassay of bovine placental lactogen using recombinant and native preparations: determination of fetal concentrations across gestation
Publication date :
2007
Journal title :
Reproduction, Fertility and Development
ISSN :
1031-3613
eISSN :
1448-5990
Publisher :
Csiro Publishing
Volume :
19
Issue :
7
Pages :
877-885
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 07 March 2009

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