Western analyses of pregnancy-associated glycoprotein family (PAG) in placental extracts of various mammals

Bella, Amina; Melo de Sousa, Noelita; Dehimi, M. L.; Watts, J.; Beckers, Jean-François

doi:10.1016/j.theriogenology.2007.08.002

Request a copy

Article (Scientific journals)

Western analyses of pregnancy-associated glycoprotein family (PAG) in placental extracts of various mammals

Bella, Amina; Melo de Sousa, Noelita; Dehimi, M. L. et al.

2007 • In Theriogenology, 68 (7), p. 1055-1066

Peer Reviewed verified by ORBi

Permalink
https://hdl.handle.net/2268/7976

DOI
10.1016/j.theriogenology.2007.08.002

PubMed
17850858

Files (1)Send to Details Statistics Bibliography Similar publications

Files

Full Text

BellaA-Therio-2007.pdf

Publisher postprint (661.34 kB)

Demander un tiré à part

Request a copy

All documents in ORBi are protected by a user license.

Send to

RIS BibTex APA Chicago Permalink X Linkedin

Details

Keywords :

pregnancy-associated glycoprotein; immunoblotting; ammonium sulfate precipitation; placenta; yolk sac

Abstract :

[en] The present study was conducted in order to analyze the inummoreactivity, of placental extracts of several animal species and humans against the following three groups of PAG antisera: anti-boPAG-1 (R#497), -boPAG-II (R#435), and -caPAG (R#706). Placental proteins were obtained after extraction at neutral pH, followed by ammonium sulfate (A.S.) precipitation, dialysis, and lyophilization. The immunoreactivity of different placental extracts was revealed by the use of monodimensional SDS-PAGE, followed by blotting on nitrocellulose membrane and the identification of immunoreactive proteins after incubation with PAG antisera (Western blot technique). A strong immunoreactivity of proteins from synepitheliochorial placenta (cattle, sheep, goat, bison, buffalo, and deer) was demonstrated in both 20-50% and 50-80% A.S. fractions using the three antisera. Proteins from species with epitheliochorial placenta presented variable profiles of detected PAG-like proteins: in the sow, many inummoreactive forms were revealed by antisera boPAG-I and boPAG-II, whereas in the dromedary, only two forms were revealed by anti-boPAG-II. Concerning other species, our protocols showed for the first time a cross-reaction between PAG antisera with proteins extracted from dog, alpaca, dromedary, sea lion, and human placenta. (C) 2007 Elsevier Inc. All rights reserved.

Disciplines :

Veterinary medicine & animal health

Author, co-author :

Bella, Amina ; Université de Liège - ULiège > Doct. sc. vété. (Bologne)

Melo de Sousa, Noelita ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction

Dehimi, M. L.

Watts, J.

Beckers, Jean-François ; Université de Liège - ULiège > Département de sciences fonctionnelles > Physiologie de la reproduction

Language :

English

Title :

Western analyses of pregnancy-associated glycoprotein family (PAG) in placental extracts of various mammals

Publication date :

2007

Journal title :

Theriogenology

ISSN :

0093-691X

eISSN :

1879-3231

Publisher :

Elsevier Science Inc, New York, United States - New York

Volume :

Issue :

Pages :

1055-1066

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 07 March 2009

Statistics

Number of views

115 (11 by ULiège)

Number of downloads

4 (3 by ULiège)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

Bibliography

Aschheim S. Weitere untersuchungen ueber hormone and schwangerschaft. Das vorkommen der hormone in harn der schwangeren. Arch Gynaecol 132 (1927) 179-183
Cole H.H., and Hart G.H. The potency of blood serum of mares in progressive stages of pregnancy in effecting the sexual maturity of the immature rat. Am J Physiol 93 (1930) 57-68
Van Hell H., Matthijsen R., and Homan J.D. Studies on human chorionic gonadotrophin. Purification and some physico-chemical properties. J Biol Chem 59 (1968) 89-104
Bahl P.O. Purification and physiocochemical properties. J Biol Chem 244 (1969) 567-574
Gospodarowicz D., and Papkoff H. A simple method for the isolation of mare serum gonadotrophin. Endocrinology 80 (1967) 699-702
Allen W.R., and Moor R.M. The origin of the equine endometrial cups. I. Production of PMSG by fetal trophoblast cells. J Reprod Fertil 29 (1972) 313-316
Amoroso E.C. Endocrinology of pregnancy. Br Med Bull 11 (1955) 117-125 [abstract]
Nalbandov A., and Casida L.E. Gonadotropic action of pituitaries from pregnant cows. Endocrinology 27 (1940) 559-564
Weeth H.J., and Herman H.A. A histological and histochemical study of the bovine oviducts, uterus and placenta. Res Bull Univ Miss Agric Exp (1952) 501
Foote W.C., and Kaushik D.K. LH activity in bovine reproductive tissue. J Anim Sci 22 (1963) 857-858
Beckers J.F., Dewulf M., Verstegen J., Wouters-Ballman P., and Ectors F. Isolation of a bovine chorionic gonadotropin (bCG). Theriogenology 29 (1988) 218 [abstract]
Xie S., Low B.G., Nagel R.J., Beckers J.F., and Roberts R.M. A novel glycoprotein of the aspartic proteinase gene family expressed in bovine placental trophectoderm. Biol Reprod 51 (1994) 1145-1153
Xie S., Low R.C., Nagel R.J., Kramer K.K., Anthony R.V., Zoli A.P., et al. Identification of the major pregnancy-specific antigens of cattle and sheep as inactive members of the aspartic proteinase family. Proc Natl Acad Sci USA 88 (1991) 10247-10251
Butler J.E., Hamilton W.C., Sasser R.G., Ruder C.A., Hass G.M., and Williams R.J. Detection and partial characterization of two bovine pregnancy specific proteins. Biol Reprod 26 (1982) 925-933
Sasser R.G., Ruder C.A., Ivani K.A., Butler J.E., and Hamilton W.C. Detection of pregnancy by radioimmunoassay of a novel pregnancy-specific protein in serum of cows and a profile of serum concentrations during gestation. Biol Reprod 35 (1986) 936-942
Mialon M.M., Camous S., Renand G., Martal J., and Menissier F. Peripheral concentrations of a 60 kDa pregnancy serum protein during gestation and after calving and in relationship to embryonic mortality in cattle. Reprod Nutr Dev 33 (1993) 269-282
Gogolin-Ewens K.J., Lee C.S., Moseby A.M., and Brandon M.R. Characterization of a sheep trophoblast-derived antigen first appearing at implantation. Placenta 7 (1986) 243-255
Zoli A.P., Beckers J.F., Wouters-Ballman P., Closset J., Falmagne P., and Ectors F. Purification and characterization of a bovine pregnancy-associated glycoprotein. Biol Reprod 45 (1991) 1-10
Zoli A.P., Guilbault L.A., Delahaut P., Benitez Ortiz W., and Beckers J.F. Radioimmunoassay of a bovine pregnancy-associated glycoprotein in serum: its application for pregnancy diagnosis. Biol Reprod 46 (1992) 83-92
Xie S., Green J.A., Bao B., Bixby J.B., Szafranska B., Demartini J.C., et al. The diversity and evolutionary relationships of the pregnancy-associated glycoproteins, an aspartic proteinase subfamily consisting of many trophoblast-expressed genes. Proc Natl Acad Sci USA 94 (1997) 12809-12816
Green J.A., Xie S., Quan X., Bao B., Gan X., Mathialagan N., et al. Pregnancy-associated glycoproteins exhibit spatially and temporally distinct expression patterns during pregnancy. Biol Reprod 62 (2000) 1624-1631
Garbayo J.M., Green J.A., Manikkam M., Beckers J.F., Kiesling D.O., Ealy A.D., et al. Caprine pregnancy-associated glycoproteins (PAG): their cloning, expression, and evolutionary relationship to other PAG. Mol Reprod Dev 57 (2000) 311-322
Garbayo JM, Serrano B, Lopez-Gatius F. Identification of novel pregnancy-associated glycoproteins (PAG) expressed by the peri-implantation conceptus of domestic ruminants. Anim Reprod Sci 2007, doi:10.1016/j.anireprosci.2006.12.002.
Vawter GA, Parks TE, Green JA, Ealy AD, Killian G. Nucleotide sequence for pregnancy-associated glycoproteins from white-tailed deer (Odocoileus virginiatus). Direct submission to GenBank. 2004 [Accession numbers Q6R6N3 to Q6R6N9 and Q6R6P0 to Q6R6P2].
Hughes A.L., Green J.A., Garbayo J.M., and Roberts M.R. Adaptive diversification within a large family of recently duplicated, placentally expressed genes. Proc Natl Acad Sci USA 97 (2000) 3319-3323
Hughes A.L., Green J.A., Piontkivska H., and Roberts R.M. Aspartic proteinase phylogeny and the origin of pregnancy-associated glycoproteins. Mol Biol Evol 20 (2003) 1940-1945
Wooding F.B., Roberts R.M., and Green J.A. Light and electron microscope immunocytochemical studies of the distribution of pregnancy associated glycoproteins (PAGs) throughout pregnancy in the cow: possible functional implications. Placenta 26 (2005) 807-827
Szafranska B., Majewska M., and Panasiewicz G. N-glycodiversity of the pregnancy-associated glycoprotein family (PAG) produced in vitro by trophoblast and trophectoderm explants during implantation, placentation and advanced pregnancy in the pig. Reprod Biol 4 (2004) 67-89
Szafranska B., Panasiewicz G., and Majewska M. Porcine pregnancy-associated glycoprotein family (pPAGs) as in vitro-produced chorionic ligands for luteal and uterine gonadotropin receptors. Reprod Biol 6 (2006) 105-111
Szafranska B., Xie S., Green J., and Roberts R.M. Porcine pregnancy-associated glycoproteins: new members of the aspartic proteinase gene family expressed in the trophectoderm. Biol Reprod 53 (1995) 21-28
Panasiewicz G., Majewska M., and Szafranska B. Trophoblastic cDNA cloning of porcine pregnancy-associated glycoprotein genes (pPAG) and in silico analysis of coded polypeptide precursors. Reprod Biol 4 (2004) 131-141
Szafranska B., Miura R., Ghosh D., Ezashi T., Xie S., Roberts R.M., et al. Gene for porcine pregnancy-associated glycoprotein 2 (pPAG2): its structural organization and analysis of its promoter. Mol Reprod Dev 60 (2001) 137-146
Green J.A., Xie S., Szafranska B., Gan X., Newman A.G., McDowell K., et al. Identification of a new aspartic proteinase expressed by the outer chorionic cell layer of the equine placenta. Biol Reprod 60 (1999) 1069-1077
Gan X., Xie S., Green J.A., and Roberts R.M. Identification of transcripts for pregnancy associated glycoprotein (PAG) in Carnivora and Perissodactyla. Biol Reprod 56 (1997) 431 [abstract]
Szafranska B., Panasiewicz G., Majewska M., and Beckers J.F. Chorionic expression of heterogeneous products of the PAG (pregnancy-associated glycoprotein) gene family secreted in vitro throughout embryonic and foetal development in the pig. Reprod Nutr Dev 43 (2003) 497-516
Majewska M., Panasiewicz G., Dabrowski M., Gizejewski Z., Beckers J.F., and Szafranska B. Multiple forms of pregnancy-associated glycoproteins released in vitro by porcine chorion or placentomal and interplacentomal explants of wild and domestic ruminants. Reprod Biol 5 (2005) 185-203
Szafranska B., and Panasiewicz G. The placental expression of the porcine pregnancy-associated glycoprotein (pPAG) gene family examined in situ and in vitro. Anim Reprod Sci 72 (2002) 95-113
El Amiri B., Sousa N.M., Mecif K., Desbuleux H., Banga-Mboko H., and Beckers J.F. Double radial immunodiffusion as a tool to identify pregnancy-associated glycoproteins in ruminant and nonruminant placenta. Theriogenology 59 (2003) 1291-1301
Evans H.E., and Sack W.O. Prenatal development of domestic and laboratory mammals: growth curves, external features and selected references. Anat Histol Embryol 2 (1973) 11-45
Martal J., and Haddad B. Physiologie et endocrinologie placentaires. In: Thibault C., and Levasseur M.C. (Eds). INRA Editions (2001), Ellipses p. 505-32
Vaitukaitis J., Robbins J.B., Nieschlag E., and Ross G.T. A method for producing specific antisera with small doses of immunogen. J Clin Endocrinol Metab 33 (1971) 988-991
Garbayo J.M., Remy B., Alabart J.L., Folch J., Wattiez R., Falmagne P., et al. Isolation and partial characterization of a pregnancy-associated glycoprotein family from the goat placenta. Biol Reprod 58 (1998) 109-115
Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
Towbin H., Stchelin T., and Gordon J. Electrophoresis transfer of protein from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76 (1979) 4350-4354
Kumar S., and Hedges S.B. A molecular timescale for vertebrate evolution. Nature 392 (1998) 917-920
Kageyama T., Tanabe K., and Koiwai O. Structure and development of rabbit pepsinogens. Stage-specific zymogens, nucleotide sequences of cDNAs, molecular evolution, and gene expression during development. J Biol Chem 265 (1990) 17031-17038
Chen X., Rosenfeld C.S., Roberts R.M., and Green J.A. An aspartic proteinase expressed in the yolk sac and neonatal stomach of the mouse. Biol Reprod 65 (2001) 1092-1101
Kageyama T., Ichinose M., Tsukada-Kato S., Omata M., Narita Y., Moriyama A., et al. Molecular cloning of neonate/infant-specific pepsinogens from rat stomach mucosa and their expressional change during development. Biochem Biophys Res Commun 267 (2000) 806-812
Kageyama T. Pepsinogens, progastricsins, and prochymosins: structure, function, evolution, and development. Cell Mol Life Sci. 59 (2002) 288-306 [review]
Drew M.L., Alexander B.M., and Sasser R.G. Pregnancy determination by use of pregnancy-specific protein B radioimmunoassay in llamas. J Am Vet Med Assoc 207 (1995) 217-219
Garbayo JM, Serrano B, Aguilar B, Lopez-Gatius F. Identification of pregnancy-associated glycoproteins (PAGs) expressed by preimplantation embryos of domestic ruminants. Direct submission to GenBank, 2005 [Acc. No.: AY911498 for boPAG22].
Xie S., Green J.A., Bao B., Beckers J.F., Valdez K.E., Hakami L., et al. Multiple pregnancy-associated glycoproteins are secreted by day 100 ovine placental tissue. Biol Reprod 57 (1997) 1384-1393
Klisch K., Sousa N.M., Beckers J.F., Leiser R., and Pich A. Pregnancy associated glycoprotein-1, -6, -7, and -17 are major products of bovine binucleate trophoblast giant cells at mid pregnancy. Mol Reprod Dev 71 (2005) 453-460
El Amiri B., Remy B., Sousa N.M., Joris B., Gerardin-Otthiers N., Perenyi Z., et al. Isolation and partial characterization of three pregnancy-associated glycoproteins from the ewe placenta. Mol Reprod Dev 64 (2003) 199-206
El Amiri B., Remy B., De Sousa N.M., and Beckers J.F. Isolation and characterization of eight pregnancy-associated glycoproteins present at high levels in the ovine placenta between day 60 and day 100 of gestation. Reprod Nutr Dev 44 (2004) 69-181
Huang F., Cockrell D.C., Stephenson T.R., Noyes J.H., and Sasser R.G. Isolation, purification, and characterization of pregnancy-specific protein B from elk and moose placenta. Biol Reprod 61 (1999) 1056-1061
Barbato O., Sousa N.M., Klisch K., Clerget E., Debenedetti A., Barile V.L., et al. Isolation of PAG from buffalo (Bubalus bubalis) placenta. Reproduction 33 Suppl (2006) P020 [abstract]
Klisch K., and Leiser R. In bovine binucleate trophoblast giant cells, pregnancy-associated glycoproteins and placental prolactin-related protein-I are conjugated to asparagine-linked N-acetylgalactosaminyl glycans. Histochem Cell Biol 119 (2003) 211-217
Atkinson Y.H., Gogolin-Ewens K.J., Hounsel E.F., Davies M.J., Brandon M.R., and Seamark R.F. Characterization of placentation-specific binucleate cell glycoproteins possessing a novel carbohydrate. J Biol Chem 268 (1993) 26679-26685
Buff S., Fontbonne A., Lopez P., Rauer M., and Creavat D. Circulation relaxin concentrations in pregnant and non-pregnant bitches: evaluation of a new enzyme immunoassay for determination of pregnancy. J Reprod Fertil 57 Suppl (2001) 187-191
Hobson B.M., and Wide L. Gonadotrophin in the term placenta of the dolphin (Tursiops truncatus), the Californian sea lion (Zalophus californianus), the grey seal (Halichoerus grypus) and man. J Reprod Fertil 76 (1986) 637-644
Rosen S.W. New placental proteins: chemistry, physiology and clinical use. Placenta 7 (1986) 575-594