Reference : Western analyses of pregnancy-associated glycoprotein family (PAG) in placental extra...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/7976
Western analyses of pregnancy-associated glycoprotein family (PAG) in placental extracts of various mammals
English
Bella, Amina mailto [Université de Liège - ULg > > > Doct. sc. vété. (Bologne)]
Melo de Sousa, Noelita mailto [Université de Liège - ULg > Département de sciences fonctionnelles > Physiologie de la reproduction >]
Dehimi, M. L. [> > > >]
Watts, J. [> > > >]
Beckers, Jean-François mailto [Université de Liège - ULg > Département de sciences fonctionnelles > Physiologie de la reproduction >]
2007
theriogenology
Elsevier Science Inc
68
7
1055-1066
Yes (verified by ORBi)
International
0093-691X
New York
[en] pregnancy-associated glycoprotein ; immunoblotting ; ammonium sulfate precipitation ; placenta ; yolk sac
[en] The present study was conducted in order to analyze the inummoreactivity, of placental extracts of several animal species and humans against the following three groups of PAG antisera: anti-boPAG-1 (R#497), -boPAG-II (R#435), and -caPAG (R#706). Placental proteins were obtained after extraction at neutral pH, followed by ammonium sulfate (A.S.) precipitation, dialysis, and lyophilization. The immunoreactivity of different placental extracts was revealed by the use of monodimensional SDS-PAGE, followed by blotting on nitrocellulose membrane and the identification of immunoreactive proteins after incubation with PAG antisera (Western blot technique). A strong immunoreactivity of proteins from synepitheliochorial placenta (cattle, sheep, goat, bison, buffalo, and deer) was demonstrated in both 20-50% and 50-80% A.S. fractions using the three antisera. Proteins from species with epitheliochorial placenta presented variable profiles of detected PAG-like proteins: in the sow, many inummoreactive forms were revealed by antisera boPAG-I and boPAG-II, whereas in the dromedary, only two forms were revealed by anti-boPAG-II. Concerning other species, our protocols showed for the first time a cross-reaction between PAG antisera with proteins extracted from dog, alpaca, dromedary, sea lion, and human placenta. (C) 2007 Elsevier Inc. All rights reserved.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/7976
10.1016/j.theriogenology.2007.08.002

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