|Reference : Cystatin C is a reliable marker for estimation of glomerular filtration rate in renal tr...|
|Scientific journals : Article|
|Human health sciences : Laboratory medicine & medical technology|
|Cystatin C is a reliable marker for estimation of glomerular filtration rate in renal transplantation: validation of a new turbidimetric assay using monospecific sheep antibodies|
|Bargnoux, A. S. [> >]|
|Cavalier, Etienne [Université de Liège - ULg > > Chimie médicale >]|
|Cristol, J. P. [> >]|
|Simon, N. [> >]|
|Dupuy, A. M. [> >]|
|Garrigue, V. [> >]|
|Delanaye, Pierre [Université de Liège - ULg > > Néphrologie >]|
|Mourad, G. [> >]|
|Clinical Chemistry & Laboratory Medicine|
|Walter de Gruyter|
|Yes (verified by ORBi)|
|[en] Cystatin C ; renal transplantation ; glomerular filtration rate ; predictive equation|
|[en] Background: The potential use of Cystatin C was recently assessed in kidney transplantation.
A new particle-enhanced turbidimetric immunoassay (PETIA) that uses sheep antibodies
(Binding Site Human Cystatin C immunoassay) has been developed. Analytical performance
of this new assay was evaluated. Clinical relevance was determined by comparison with a
reference method in a cohort of kidney transplant patients.
Patients and methods: First, the analytical performance of the Binding Site cystatin C kit
was tested on SPAPLUS® and Hitachi® analyzers. Second, a comparison study was performed
using SPAPLUS® analyzer against two other cystatin C methods (the Siemens-PENIA method
on BNII® and the Dako-PETIA application on Olympus AU640®). Third, the glomerular
filtration rate (GFR) was estimated using several predictive cystatin C- and creatinine-based
equations and compared to GFR measured by an isotopic method (99mTc-DTPA). These
predictive algorithms were analyzed with respect to bias, precision and accuracy.
Results: Total intra-assay and inter-assay coefficients of variation were below 5%. Values
obtained with the SPAPLUS® correlated with the Siemens-PENIA and the Dako-PETIA
methods. The creatinine and cystatin C-based equation allowed reliable assessment of GFR in
our population of renal transplantation.
Conclusions: The use of algorithms based on cystatin C and creatinine could provide a
reliable estimate of GFR in kidney transplantation.
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