Reference : 1,6-AnhMurNAc derivatives for assay development of amidase AmiD.
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Physical, chemical, mathematical & earth Sciences : Chemistry
http://hdl.handle.net/2268/79276
1,6-AnhMurNAc derivatives for assay development of amidase AmiD.
English
Mercier, Frédéric mailto [Université de Liège - ULg > > Centre de recherches du cyclotron >]
Zervosen, Astrid mailto [Université de Liège - ULg > > Centre de recherches du cyclotron >]
Teller, Nathalie [Université de Liège - ULg > > >Centre de recherche du Cyclotron > > >]
Frère, Jean-Marie mailto [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
Herman, Raphaël mailto [Université de Liège - ULg > > Centre d'ingénierie des protéines >]
Pennartz, Anne [Université de Liège - ULg > > > Centre d'ingénierie des protéines > > >]
Joris, Bernard mailto [Université de Liège - ULg > Département des sciences de la vie > Physiologie et génétique bactériennes - Centre d'ingénierie des protéines >]
Luxen, André mailto [Université de Liège - ULg > Département de chimie (sciences) > Chimie organique de synthèse - Centre de recherches du cyclotron >]
2010
Bioorganic & Medicinal Chemistry
Elsevier Science
18
21
7422-31
Yes (verified by ORBi)
International
0968-0896
1464-3391
Oxford
United Kingdom
[en] amidase AmiD ; anhydro-muramic acid
[en] Various peptidoglycan fragments were synthesized from two anhydro-muramic acid derivatives protected with a Bn or a PMB group at the 4th position, in homogenate phase or on a solid support. In order to facilitate HPLC detection, a chromophoric group was attached to the peptide chain. The periplasmic amidase sAmiD of Escherichia coli was used to cleave the amide bond between the lactyl group of the MurNAc and the alpha-amino group of L-Ala where the peptide chain was at least a dipeptide (L-Ala-gamma-D-Glu) amidated by benzylamine on the gamma-carboxyl group of D-Glu. In the presence of a tripeptide chain (L-Ala-gamma-D-Glu-L-Lys) or a tetrapeptide chain (L-Ala-gamma-D-Glu-m-A(2)pm-D-Ala) higher hydrolysis rates were observed. We have also demonstrated that the presence of TNB on the epsilon-amino group of L-Lys only has a small influence on the hydrolysis capacity of sAmiD.
Centre de Recherches du Cyclotron - CRC ; Centre d'Ingénierie des Protéines - CIP
Belgian Program on Interuniversity Poles of Attraction initiated by the Belgian state
Researchers ; Professionals
http://hdl.handle.net/2268/79276
10.1016/j.bmc.2010.09.010
Copyright (c) 2010 Elsevier Ltd. All rights reserved.

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