Paper published in a book (Scientific congresses and symposiums)
Antimicrobial Susceptibilities of recent clinical isolates of group B streptococci agalactiae from Belgium
MELIN, Pierrette; Maquet, Julie; Rodriguez Cuns, Grisel et al.
2003In American Society of Microbiology (Ed.) Program and Abstracts of the 43rd Intersciences Conference on Antimicrobial Agents and Chemotherapy
Peer reviewed
 

Files


Full Text
GBS AST and Pen tolerance ICAAC 2003.pdf
Publisher postprint (284.32 kB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Group B streptococci; Antimicrobial susceptibility; clinical isolates; Belgium
Abstract :
[en] Background: : GBS cause severe infections in neonates, pregnant women and other adults. Empiric therapy is usually started before susceptibility results are available. Early neonatal diseases can be prevented with intrapartum antibiotic prophylaxis based on accurate susceptibility surveillance data. A previous Belgian study showed an increase of 3 to 10 % R to erythromycin (EM) through the 1990s. Methods: 187 GBS isolates consecutively received at the reference laboratory between 2001 to March 2003 were from 73 neonates (52 early-onset and 21 late-onset diseases), 52 adults and 62 from pregnant women’s vagina. MICs of penicillin (PG), EM, clindamycin (CM) and gentamicin (GM) were determined with Etest. PG MBCs were also determined by inactivating the drug in MIC plates using betalactamase. EM resistant (R) isolates were tested by the CM + EM double disk to determine macrolide R phenotypes. Results: All strains were susceptible (S) to PG and no tolerance was observed with MBCs falling within 2 dilutions of MICs. 19.2% of isolates were R to EM, with significantly more R isolates from adults (30.8%; p <0.01) and serotype V (46.8%; p <0,001). 80% had the MLSB phenotype (R to EM and CM), 16 were constitutive and 12 inducible. The M phenotype (R to EM and S to CM) was seen in 7 (20%) of isolates. Less than 10% of isolates were inhibited by GM MIC of <=64 mg/L, 83.6% by 128-256 mg/L and 2.9% by >/=512 mg/L. Non typable strains were more R to GM (p <0.01). Conclusions: 1) PG remained active against all isolates and no tolerance was seen. 2) Prevalence of R to macrolides had increased since 1999, particularly in adult isolates and serotype V. 3) Intermediate to high level R to GM was seen and potential synergy of PG + GM should be investigated. 4) R surveillance is mandatory to guide prophylaxis and treatment of serious GBS infections.
Disciplines :
Immunology & infectious disease
Laboratory medicine & medical technology
Author, co-author :
MELIN, Pierrette  ;  Centre Hospitalier Universitaire de Liège - CHU > Microbiologie médicale
Maquet, Julie
Rodriguez Cuns, Grisel;  Universitad de la Republica, Montevideo, Uruguay
HAYETTE, Marie-Pierre ;  Centre Hospitalier Universitaire de Liège - CHU > Microbiologie médicale
CHRISTIAENS, Geneviève ;  Centre Hospitalier Universitaire de Liège - CHU > Direction médicale
De Mol, Patrick ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Microbiologie médicale et virologie médicale
Language :
English
Title :
Antimicrobial Susceptibilities of recent clinical isolates of group B streptococci agalactiae from Belgium
Publication date :
September 2003
Event name :
43rd Intersciences Conference on Antimicrobial Agents and Chemotherapy (ICAAC)
Event organizer :
American Society of Microbiology
Event place :
Chicago, United States
Event date :
Du 13 au 17 septembre 2003
Audience :
International
Main work title :
Program and Abstracts of the 43rd Intersciences Conference on Antimicrobial Agents and Chemotherapy
Editor :
American Society of Microbiology
Publisher :
American Society for Microbiology (ASM), w, United States
Pages :
Abstract du poster C2-81
Peer reviewed :
Peer reviewed
Available on ORBi :
since 28 February 2011

Statistics


Number of views
71 (4 by ULiège)
Number of downloads
53 (3 by ULiège)

Bibliography


Similar publications



Contact ORBi