Reference : Fermentative hydrogen production by Clostridium butyricum CWBI1009 and Citrobacter fr...
Scientific journals : Article
Life sciences : Microbiology
Life sciences : Biotechnology
http://hdl.handle.net/2268/78754
Fermentative hydrogen production by Clostridium butyricum CWBI1009 and Citrobacter freundii CWBI952 in pure and mixed cultures
English
Beckers, Laurent* mailto [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
Hiligsmann, Serge* mailto [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
Hamilton, Christopher [Université de Liège - ULg > > Centre Wallon de biologie industrielle >]
Masset, Julien mailto [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles >]
Thonart, Philippe mailto [Université de Liège - ULg > Département des sciences de la vie > Biochimie et microbiologie industrielles - Bio-industries >]
* These authors have contributed equally to this work.
2010
Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE]
Presses Agronomiques de Gembloux
14
S2
2e journée de réflexion de l'EDT GEPROC : Génie des procédés appliqué aux bio-industries. 16 décembre 2009, Faculté universitaire des Sciences agronomiques, Gembloux, Belgique
541-548
Yes (verified by ORBi)
International
1370-6233
Gembloux
Belgique
[en] Clostridium butyricum ; Citrobacter freundii ; biohydrogen ; mixed culture ; biochemical hydrogen potential ; batch ; starch ; glucose
[en] This paper investigates the biohydrogen production by two mesophilic strains, a strict anaerobe (Clostridium butyricum CWBI1009) and a facultative anaerobe (Citrobacter freundii CWBI952). They were cultured in pure and mixed cultures in serum bottles with five different carbon sources. The hydrogen yields of pure C. freundii cultures ranged from 0.09 molH2.molhexose-1 (with sucrose) to 0.24 molH2.molhexose-1 (with glucose). Higher yields were obtained by the pure cultures of Cl. butyricum ranging from 0.44 molH2.molhexose-1 (with sucrose) to 0.69 molH2.molhexose-1 (with lactose). This strain also fermented starch whereas C. freundii did not. However, it consumed the other substrates faster and produced hydrogen earlier than Cl. butyricum. This ability has been used to promote the growth conditions of Cl. butyricum in co-culture with C. freundii, since Cl. butyricum is extremely sensitive to the presence of oxygen which strongly inhibits H2 production. This approach could avoid the addition of any expensive reducing agents in the culture media such as L-cysteine since C. freundii consumes the residual oxygen. Thereafter, co-cultures with glucose and starch were investigated: hydrogen yields decreased from 0.53 molH2.molhexose-1 for pure Cl. butyricum cultures to 0.38 molH2.molhexose
-1 for mixed culture with glucose but slightly increased with starch (respectively 0.69 and 0.73 molH2.molhexose-1). After 48 h of fermentation, metabolites analysis confirmed with microbial observation, revealed that the cell concentration of C. freundii dramatically decreased or was strongly inhibited by the development of Cl. butyricum.
Centre Wallon de Biologie Industrielle
Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS ; Action de Recherches concertées ARC 07/12 04- ULg- Communauté française ; Fonds pour la formation à la Recherche dans l'Industrie et dans l'Agriculture (Communauté française de Belgique) - FRIA ; Région wallonne : Direction générale des Technologies, de la Recherche et de l'Energie - DGTRE
Etude de la production d'hydrogène par les bactéries anaérobies chimiotrophes (dark-fermentation)
Researchers ; Professionals ; Students ; General public
http://hdl.handle.net/2268/78754
http://www.bib.fsagx.ac.be/base/text/v14ns2/541.pdf
http://www.microh2.ulg.ac.be
http://cwbi.fsagx.ac.be

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