Reference : Dendritic cells genetically engineered to express IL-10 induce long-lasting antigen-spec...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/7836
Dendritic cells genetically engineered to express IL-10 induce long-lasting antigen-specific tolerance in experimental asthma.
English
Henry, E. [ > > ]
Desmet, Christophe mailto [Université de Liège - ULg > Département de sciences fonctionnelles > GIGA-R : Biochimie et biologie moléculaire >]
Garze, V. [> > > >]
Fievez, Laurence mailto [Université de Liège - ULg > Département de sciences fonctionnelles > GIGA-R : Biochimie et biologie moléculaire >]
Bedoret, Denis [Université de Liège - ULg > Département de sciences fonctionnelles > Physiologie >]
Heirman, C. [> > > >]
Faisca, Pedro [> > > >]
Jaspar, F. [ > > ]
Gosset, Ph [> > > >]
Jacquet, A. [> > > > >]
Desmecht, Daniel mailto [Université de Liège - ULg > Département de morphologie et pathologie > Pathologie spéciale et autopsies >]
Thielemans, Kris [> > > >]
Lekeux, Pierre mailto [Université de Liège - ULg > Département de sciences fonctionnelles > Physiologie - Doyen de la Faculté de Médecine vétérinaire >]
Moser, M. [> > > >]
Bureau, Fabrice mailto [Université de Liège - ULg > Département de sciences fonctionnelles > GIGA-R : Biochimie et biologie moléculaire >]
2008
Journal of Immunology
American Association of Immunologists
181
10
7230-7242
Yes (verified by ORBi)
International
0022-1767
1550-6606
Baltimore
MD
[en] Adoptive Transfer ; Animals ; Antigens/immunology ; Apoptosis/immunology ; Asthma/immunology/metabolism/therapy ; Cells, Cultured ; Dendritic Cells/immunology/metabolism ; Disease Models, Animal ; Female ; Flow Cytometry ; Genetic Engineering/methods ; Immune Tolerance ; Immunotherapy/methods ; Interleukin-10/genetics/metabolism ; Mice ; Mice, Knockout ; Ovalbumin/immunology ; Reverse Transcriptase Polymerase Chain Reaction ; T-Lymphocytes, Regulatory/immunology ; Transduction, Genetic
[en] Dendritic cells (DCs) are professional APCs that have a unique capacity to initiate primary immune responses, including tolerogenic responses. We have genetically engineered bone marrow-derived DCs to express the immunosuppressive cytokine IL-10 and tested the ability of these cells to control experimental asthma. A single intratracheal injection of OVA-pulsed IL-10-transduced DCs (OVA-IL-10-DCs) to naive mice before OVA sensitization and challenge prevented all of the cardinal features of airway allergy, namely, eosinophilic airway inflammation, airway hyperreactivity, and production of mucus, Ag-specific Igs, and IL-4. OVA-IL-10-DCs also reversed established experimental asthma and had long-lasting and Ag-specific effects. We furthermore showed, by using IL-10-deficient mice, that host IL-10 is required for mediating the immunomodulatory effects of OVA-IL-10-DCs and demonstrated a significant increase in the percentage of OVA-specific CD4(+)CD25(+)Foxp3(+)IL-10(+) regulatory T cells in the mediastinal lymph nodes of OVA-IL-10-DC-injected mice. Finally, adoptive transfer of CD4(+) mediastinal lymph node T cells from mice injected with OVA-IL-10-DCs protected OVA-sensitized recipients from airway eosinophilia upon OVA provocation. Our study describes a promising strategy to induce long-lasting Ag-specific tolerance in airway allergy.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/7836

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