Animals; Gene Expression Regulation, Developmental; In Situ Hybridization; Kidney/embryology/metabolism; Organ Specificity; Organogenesis; Sodium-Phosphate Cotransporter Proteins, Type III/genetics; Xenopus Proteins/genetics; Xenopus laevis/embryology/genetics/metabolism; Zebrafish/embryology/genetics/metabolism; Zebrafish Proteins/genetics
Abstract :
[en] The embryonic pronephric kidneys of Xenopus and zebrafish serve as models to study vertebrate nephrogenesis. Recently, multiple subdomains within the Xenopus pronephros have been defined based on the expression of several transport proteins. In contrast, very few studies on the expression of renal transporters have been conducted in zebrafish. We have recently shown that the anterior and posterior segments of the zebrafish pronephric duct may correspond to the proximal tubule and distal tubule/duct compartments of the Xenopus and higher vertebrate pronephros, respectively. Here, we report the embryonic expression pattern of the Na(+)/PO(4) cotransporter SLC20A1 (PiT1/Glvr-1) gene encoding a type III sodium-dependent phosphate cotransporter in Xenopus and zebrafish. In Xenopus, SLC20A1 mRNA is expressed in the somitic mesoderm and lower level of expression is detected in the neural tube, eye, and neural crest cells. From stage 25, SLC20A1 is also detectable in the developing pronephros where expression is restricted to the late portion of the distal pronephric tubules. In zebrafish, SLC20A1 is transcribed from mid-somitogenesis in the anterior part of the pronephros where its expression corresponds to the rostral portion of the expression of other proximal tubule-specific markers. Outside the pronephros, lower level of SLC20A1 expression is also observed in the posterior cardinal and caudal veins. Based on the SLC20A1 expression domain and that of other transporters, four segments have been defined within the zebrafish pronephros. Together, our data reveal that the zebrafish and Xenopus pronephros have non-identical proximo-distal organizations.
Research center :
Giga-Development and Stem Cells - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Nichane, Massimo; Université Libre de Bruxelles - ULB > Institut de Biologie et de Médecine Moléculaires > Laboratoire d’Embryologie Moléculaire
Van Campenhout, Claude; Université Libre de Bruxelles - ULB > Institut de Biologie et de Médecine Moléculaires > Laboratoire d’Embryologie Moléculaire
Pendeville, Helene; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Voz, Marianne ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Bellefroid, Eric J.; Université Libre de Bruxelles - ULB > Institut de Biologie et de Médecine Moléculaires > Laboratoire d’Embryologie Moléculaire
Language :
English
Title :
The Na+/PO4 cotransporter SLC20A1 gene labels distinct restricted subdomains of the developing pronephros in Xenopus and zebrafish embryos.
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