ORBi: Mouton Veronique - Heart 6-phosphofructo-2-kinase activation by insulin requires PKB (protein kinase B), but not SGK3 (serum- and glucocorticoid-induced protein kinase 3).

Reference : Heart 6-phosphofructo-2-kinase activation by insulin requires PKB (protein kinase B), bu...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/78031

Title :	Heart 6-phosphofructo-2-kinase activation by insulin requires PKB (protein kinase B), but not SGK3 (serum- and glucocorticoid-induced protein kinase 3).
Language :	English
Author, co-author :	Mouton, Veronique [> > > >]
	Toussaint, Louise [Université de Liège - ULg > Chimie et bio-industries > Centre de Bio. Fond. - Section de Biologie cell. et moléc. >]
	Vertommen, Didier [> > > >]
	Gueuning, Marie-Agnes [> > > >]*
	Maisin, Liliane [> > > >]
	Havaux, Xavier [> > > >]
	Sanchez-Canedo, Cossette [> > > >]
	Bertrand, Luc [> > > >]
	Dequiedt, Franck [Université de Liège - ULg > > GIGA-Research - Centre de Bio. Fond. - Section de Biologie cell. et moléc. >]
	Hemmings, Brian A [> > > >]
	Hue, Louis [> > > >]
	Rider, Mark H [> > > >]
Publication date :	2010
Journal title :	Biochemical Journal
Publisher :	Portland Press
Volume :	431
Issue/season :	2
Pages :	267-75
Audience :	International
ISSN :	0264-6021
e-ISSN :	1470-8728
City :	London
Country :	United Kingdom
Keywords :	[en] Animals ; Binding Sites ; Cattle ; Cell Line ; Enzyme Activation/drug effects ; Humans ; Insulin/pharmacology ; Male ; Mice ; Mice, Transgenic ; Mutation/genetics ; Myocardium/cytology/enzymology ; Myocytes, Cardiac/drug effects/enzymology ; Organ Specificity/drug effects ; Phosphofructokinase-2/metabolism ; Protein Kinase Inhibitors/pharmacology ; Protein-Serine-Threonine Kinases/metabolism ; Proto-Oncogene Proteins c-akt/antagonists & inhibitors/deficiency/metabolism ; Rats ; Rats, Wistar ; Substrate Specificity/drug effects
Abstract :	[en] On the basis of transfection experiments using a dominant-negative approach, our previous studies suggested that PKB (protein kinase B) was not involved in heart PFK-2 (6-phosphofructo2-kinase) activation by insulin. Therefore we first tested whether SGK3 (serum- and glucocorticoid-induced protein kinase 3) might be involved in this effect. Treatment of recombinant heart PFK-2 with [gamma-32P]ATP and SGK3 in vitro led to PFK-2 activation and phosphorylation at Ser466 and Ser483. However, in HEK-293T cells [HEK (human embryonic kidney)-293 cells expressing the large T-antigen of SV40 (simian virus 40)] co-transfected with SGK3 siRNA (small interfering RNA) and heart PFK-2, insulin-induced heart PFK-2 activation was unaffected. The involvement of PKB in heart PFK-2 activation by insulin was re-evaluated using different models: (i) hearts from transgenic mice with a muscle/heart-specific mutation in the PDK1 (phosphoinositide-dependent protein kinase 1)-substrate-docking site injected with insulin; (ii) hearts from PKBbeta-deficient mice injected with insulin; (iii) freshly isolated rat cardiomyocytes and perfused hearts treated with the selective Akti-1/2 PKB inhibitor prior to insulin treatment; and (iv) HEK-293T cells co-transfected with heart PFK-2, and PKBalpha/beta siRNA or PKBalpha siRNA, incubated with insulin. Together, the results indicated that SGK3 is not required for insulin-induced PFK-2 activation and that this effect is likely mediated by PKBalpha.
Target :	Researchers ; Professionals ; Students
Permalink :	http://hdl.handle.net/2268/78031
DOI :	10.1042/BJ20101089

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