Article (Scientific journals)
Structure of a Zn2+-containing D-alanyl-D-alanine-cleaving carboxypeptidase at 2.5 A resolution.
Dideberg, O.; Charlier, Paulette; Dive, Georges et al.
1982In Nature, 299 (5882), p. 469-470
Peer Reviewed verified by ORBi
 

Files


Full Text
11-4332.pdf
Publisher postprint (980.73 kB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Carboxypeptidases/metabolism; Catalysis; Metalloproteins; Muramoylpentapeptide Carboxypeptidase/metabolism; Protein Conformation; Streptomyces/enzymology; X-Ray Diffraction; Zinc
Abstract :
[en] Bacteria possess proteases that are specific for the peptide bonds between D-alanine residues, one of which has a free alpha-carboxyl group. These D-alanyl-D-alanine peptidases catalyse carboxypeptidation and transpeptidation reactions involved in bacterial cell wall metabolism1,2, and are inactivated by beta-lactam antibiotics. We have now elucidated the structure, at 2.5 Å resolution, of the penicillin-resistant Zn2+-containing D-alanyl-D-alanine peptidase of Streptomyces albus (Zn2+ G peptidase)3,4. The enzyme is shown to consist of two globular domains, connected by a single link. The N-terminal domain has three alpha-helices, and the C-terminal domain has three alpha-helices and five beta-strands. The Zn2+ ion is ligated by three histidine residues, and located in a cleft in the C-terminal domain. The mechanism of action of the enzyme may be related to that of other carboxypeptidases, which also contain functional Zn2+ ions.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Dideberg, O.;  Université de Liège - ULiège > Laboratoire de Cristallographie
Charlier, Paulette ;  Université de Liège - ULiège > Département des sciences de la vie > Cristallographie des macromolécules biologiques
Dive, Georges ;  Université de Liège - ULiège
Joris, Bernard ;  Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes
Frère, Jean-Marie ;  Université de Liège - ULiège
Ghuysen, Jean-Marie ;  Université de Liège - ULiège
Language :
English
Title :
Structure of a Zn2+-containing D-alanyl-D-alanine-cleaving carboxypeptidase at 2.5 A resolution.
Publication date :
1982
Journal title :
Nature
ISSN :
0028-0836
eISSN :
1476-4687
Publisher :
Nature Publishing Group, Basingstoke, United Kingdom
Volume :
299
Issue :
5882
Pages :
469-470
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 01 September 2011

Statistics


Number of views
218 (12 by ULiège)
Number of downloads
238 (0 by ULiège)

Scopus citations®
 
108
Scopus citations®
without self-citations
96
OpenCitations
 
97

Bibliography


Similar publications



Contact ORBi