Article (Scientific journals)
Proteins Specified by Bovine Herpesvirus Type 4: Structural Proteins of the Virion and Identification of Two Major Glycoproteins by Using Monoclonal Antibodies
Dubuisson, Jean; Boulanger, Denise; Bublot, Michel et al.
1989In Journal of General Virology, 70 (Pt 7), p. 1743-53
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Abstract :
[en] Bovine herpesvirus type 4 proteins were identified by PAGE of [35S]methionine- or [3H]glucosamine-labelled purified virions. Thirty-one monoclonal antibodies (MAbs) raised against the V. Test strain were used to identify 29 proteins, ten of which were glycosylated. All of these glycoproteins belonged to the viral envelope and a 140K non-glycosylated protein appeared to be the major nucleocapsid protein. The MAbs were classified into two groups. The first group precipitated three glycoproteins of Mr 150K, 120K and 51K. The 120K and 51K glycoproteins were linked by disulphide bonds and the 150K glycoprotein was linked to the others by non-covalent bonds. The second group precipitated a different 120K glycoprotein.
Disciplines :
Microbiology
Veterinary medicine & animal health
Author, co-author :
Dubuisson, Jean
Boulanger, Denise
Bublot, Michel
Thiry, Etienne ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Virologie, épidémiologie et pathologie des maladies virales
Pastoret, Paul-Pierre ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Département des maladies infectieuses et parasitaires
Language :
English
Title :
Proteins Specified by Bovine Herpesvirus Type 4: Structural Proteins of the Virion and Identification of Two Major Glycoproteins by Using Monoclonal Antibodies
Publication date :
July 1989
Journal title :
Journal of General Virology
ISSN :
0022-1317
eISSN :
1465-2099
Publisher :
Society for General Microbiology, United Kingdom
Volume :
70 (Pt 7)
Pages :
1743-53
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 05 March 2009

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