ORBi: Gangolf Marjorie - Thiamine Status in Humans and Content of Phosphorylated Thiamine Derivatives in Biopsies and Cultured Cells

Reference : Thiamine Status in Humans and Content of Phosphorylated Thiamine Derivatives in Biopsies...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Biochemistry, biophysics & molecular biology
	To cite this reference:	http://hdl.handle.net/2268/77473

Title :	Thiamine Status in Humans and Content of Phosphorylated Thiamine Derivatives in Biopsies and Cultured Cells
Language :	English
Author, co-author :	Gangolf, Marjorie [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique >]
	Czerniecki, Jan [ > > ]
	Radermecker, Marc [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie humaine >]
	Detry, Olivier [Université de Liège - ULg > > Chirurgie abdominale- endocrinienne et de transplantation >]
	NISOLLE, Michelle [Centre Hospitalier Universitaire de Liège - CHU > > Gynécologie-Obstétrique CHR >]
	JOUAN, Caroline [Centre Hospitalier Universitaire de Liège - CHU > > Gynécologie-Obstétrique CHR >]
	Martin, Didier [Université de Liège - ULg > Département des sciences cliniques > Neurochirurgie >]
	Chantraine, Frédéric [Université de Liège - ULg > > Gynécologie-Obstétrique CHR >]
	Lakaye, Bernard [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique >]
	Wins, Pierre [ > > ]
	Grisar, Thierry [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique >]
	Bettendorff, Lucien [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique >]
Publication date :	Oct-2010
Journal title :	PLoS ONE
Publisher :	Public Library of Science
Volume :	5
Issue/season :	10
Pages :	e13616
Peer reviewed :	Yes (verified by ORBi)
Audience :	International
e-ISSN :	1932-6203
City :	San Franscisco
Country :	CA
Keywords :	[en] Thiamine triphosphate ; adenosine thiamine triphosphate ; thiamine diphosphate ; mitochondria ; thiamine triphosphatase ; thiamine deficiency ; cardiac insufficiency ; male infertility ; aging ; embryonic tissues ; human cell lines ; cell differentiation
Abstract :	[en] Background Thiamine (vitamin B1) is an essential molecule for all life forms because thiamine diphosphate (ThDP) is an indispensable cofactor for oxidative energy metabolism. The less abundant thiamine monophosphate (ThMP), thiamine triphosphate (ThTP) and adenosine thiamine triphosphate (AThTP), present in many organisms, may have still unidentified physiological functions. Diseases linked to thiamine deficiency (polyneuritis, Wernicke-Korsakoff syndrome) remain frequent among alcohol abusers and other risk populations. This is the first comprehensive study on the distribution of thiamine derivatives in human biopsies, body fluids and cell lines. Methodology and Principal Findings Thiamine derivatives were determined by HPLC. In human tissues, the total thiamine content is lower than in other animal species. ThDP is the major thiamine compound and tissue levels decrease at high age. In semen, ThDP content correlates with the concentration of spermatozoa but not with their motility. The proportion of ThTP is higher in humans than in rodents, probably because of a lower 25-kDa ThTPase activity. The expression and activity of this enzyme seems to correlate with the degree of cell differentiation. ThTP was present in nearly all brain and muscle samples and in ~60% of other tissue samples, in particular fetal tissue and cultured cells. A low ([ThTP]+[ThMP])/([Thiamine]+[ThMP]) ratio was found in cardiovascular tissues of patients with cardiac insufficiency. AThTP was detected only sporadically in adult tissues but was found more consistently in fetal tissues and cell lines. Conclusions and Significance The high sensitivity of humans to thiamine deficiency is probably linked to low circulating thiamine concentrations and low ThDP tissue contents. ThTP levels are relatively high in many human tissues, as a result of low expression of the 25-kDa ThTPase. Another novel finding is the presence of ThTP and AThTP in poorly differentiated fast-growing cells, suggesting a hitherto unsuspected link between these compounds and cell division or differentiation.
Research units :	Giga-Neurosciences
Funders :	Fonds de la Recherche Scientifique (Communauté française de Belgique) - F.R.S.-FNRS
Target :	Researchers ; Professionals ; Students ; General public
Permalink :	http://hdl.handle.net/2268/77473
DOI :	10.1371/journal.pone.0013616
Other URL :	http://dx.plos.org/10.1371/journal.pone.0013616

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