Reference : Increase in viral load, viral integration, and gain of telomerase genes during uterine c...
Scientific journals : Article
Human health sciences : Oncology
Human health sciences : Immunology & infectious disease
http://hdl.handle.net/2268/77368
Increase in viral load, viral integration, and gain of telomerase genes during uterine cervical carcinogenesis can be simultaneously assessed by the HPV 16/18 MLPA-assay.
English
Theelen, Wendy [> > Departments of Molecular Cell Biology, > GROW - School for Oncology and Developmental Biology, Maastricht University Medical Center, Maastricht, The Netherlands; > >]
Speel, Ernst*-Jan M [> > Departments of Molecular Cell Biology, > GROW - School for Oncology and Developmental Biology, Maastricht University Medical Center, Maastricht, The Netherlands; > >]
Herfs, Michael mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Reijans, Martin [> > > >]
Simons, Guus [> > > >]
Meulemans, Els V [> > > >]
Baldewijns, Marcella M [> > > >]
Ramaekers, Frans C S [> > > >]
Somja, Joan mailto [Centre Hospitalier Universitaire de Liège - CHU > > Anatomie pathologique >]
Delvenne, Philippe mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
Hopman, Anton H N mailto [> > > >]
2010
American Journal of Pathology
American Society for Investigative Pathology
177
4
2022-33
Yes (verified by ORBi)
International
0002-9440
1525-2191
Bethesda
MD
[en] Oncogenic human papillomavirus (HPV) infection is the most important risk factor in cervical carcinogenesis cases; high viral loads, viral integration into the host genome, and gain of the telomerase-related genes, TERT and TERC, are all factors associated with progression to cancer. A recently developed multiparameter HPV 16/18 multiplex ligation-dependent probe amplification (MLPA) assay, which allows the simultaneous assessment of these factors, was applied to a series of 67 normal and (pre)malignant frozen uterine cervical samples, as well as to 91 cytological preparations, to test the ability of the MLPA assay to identify high-risk lesions on the basis of these factors. Validation was performed using quantitative PCR, the PapilloCheck and fluorescence in situ hybridization. Only 5 out of 37 normal tissue samples or low-grade cervical lesions (ie, CIN1 and condyloma) showed either an HPV16 viral load higher than 25 copies per cell, viral integration, and/or gain of one of the telomerase-related genes, whereas for the high-grade cervical lesions, one or more of these risk factors was found in 25 of 30 cases. The HPV MLPA assay showed a sensitivity of 83% and a specificity of 86% in frozen cervical specimens. Furthermore, the feasibility of the MLPA assay was shown for cytological samples, where in 57% of high-grade squamous intraepithelial lesion cases, the high-risk factors were detected using this assay.
GIGA-Research
http://hdl.handle.net/2268/77368
10.2353/ajpath.2010.090901

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