ORBi: Theelen Wendy - Increase in viral load, viral integration, and gain of telomerase genes during uterine cervical carcinogenesis can be simultaneously assessed by the HPV 16/18 MLPA-assay.

Reference : Increase in viral load, viral integration, and gain of telomerase genes during uterine c...


	Document type :	Scientific journals : Article
	Discipline(s) :	Human health sciences : Oncology Human health sciences : Immunology & infectious disease
	To cite this reference:	http://hdl.handle.net/2268/77368

Title :	Increase in viral load, viral integration, and gain of telomerase genes during uterine cervical carcinogenesis can be simultaneously assessed by the HPV 16/18 MLPA-assay.
Language :	English
Author, co-author :	Theelen, Wendy [> > Departments of Molecular Cell Biology, > GROW - School for Oncology and Developmental Biology, Maastricht University Medical Center, Maastricht, The Netherlands; > >]
	Speel, Ernst-Jan M [> > Departments of Molecular Cell Biology, > GROW - School for Oncology and Developmental Biology, Maastricht University Medical Center, Maastricht, The Netherlands; > >]*
	Herfs, Michael [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
	Reijans, Martin [> > > >]
	Simons, Guus [> > > >]
	Meulemans, Els V [> > > >]
	Baldewijns, Marcella M [> > > >]
	Ramaekers, Frans C S [> > > >]
	Somja, Joan [Centre Hospitalier Universitaire de Liège - CHU > > Anatomie pathologique >]
	Delvenne, Philippe [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
	Hopman, Anton H N [> > > >]
Publication date :	2010
Journal title :	American Journal of Pathology
Publisher :	American Society for Investigative Pathology
Volume :	177
Issue/season :	4
Pages :	2022-33
Audience :	International
ISSN :	0002-9440
e-ISSN :	1525-2191
City :	Bethesda
Country :	MD
Abstract :	[en] Oncogenic human papillomavirus (HPV) infection is the most important risk factor in cervical carcinogenesis cases; high viral loads, viral integration into the host genome, and gain of the telomerase-related genes, TERT and TERC, are all factors associated with progression to cancer. A recently developed multiparameter HPV 16/18 multiplex ligation-dependent probe amplification (MLPA) assay, which allows the simultaneous assessment of these factors, was applied to a series of 67 normal and (pre)malignant frozen uterine cervical samples, as well as to 91 cytological preparations, to test the ability of the MLPA assay to identify high-risk lesions on the basis of these factors. Validation was performed using quantitative PCR, the PapilloCheck and fluorescence in situ hybridization. Only 5 out of 37 normal tissue samples or low-grade cervical lesions (ie, CIN1 and condyloma) showed either an HPV16 viral load higher than 25 copies per cell, viral integration, and/or gain of one of the telomerase-related genes, whereas for the high-grade cervical lesions, one or more of these risk factors was found in 25 of 30 cases. The HPV MLPA assay showed a sensitivity of 83% and a specificity of 86% in frozen cervical specimens. Furthermore, the feasibility of the MLPA assay was shown for cytological samples, where in 57% of high-grade squamous intraepithelial lesion cases, the high-risk factors were detected using this assay.
Research units :	GIGA-Research
Permalink :	http://hdl.handle.net/2268/77368
DOI :	10.2353/ajpath.2010.090901

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