Reference : Detection of biomarkers of pathogenic bacteria by matrix-assisted laser desorption/io...
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Physical, chemical, mathematical & earth Sciences : Chemistry
Life sciences : Environmental sciences & ecology
http://hdl.handle.net/2268/77223
Detection of biomarkers of pathogenic bacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
English
Ruelle, Virginie [ > > ]
El Moualij, B. [ > > ]
Zorzi, Willy mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine - Département des sciences biomédicales et précliniques >]
Zorzi, Danièle mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques >]
Ledent, P. [ > > ]
Pierard, Olivier mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
Bonjean, Nicolas [ > > ]
Gillet, Marie-Claire mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie - Cytologie - Département des sciences biomédicales et précliniques >]
Heinen, Ernst mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
De Pauw, Edwin mailto [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
2005
Environmental Chemistry : Green Chemistry and Pollutants in Ecosystems
Lichtfouse, Eric
Schwarzbauer, Jan
Robert, Didier
Springer
31-42
Berlin
Germany
[en] MALDI-TOF MS ; bacteria ; biomarker ; activated sludge ; wastewater treatment ; optimization
[en] In recent years, various mass spectrometry procedures has been developed for identifying bacteria. The accuracy and speed with which data can be obtained by Matrix-assisted laser Desorption/Ionization Time-of-flight Mass Spectrometry (MALDI-TOF-MS) make this an advantageous technique for environmental monitoring. However, minor variations in the sample preparation can influence the mass spectra significantly. In the present study, we have introduced a procedure to prepare bacteria by microextraction and we have optimized experimental parameters for rapid identification by MALDI-TOF-MS of whole bacterial cells isolated from environmental samples such as wastewater and soil.
Centre Interfacultaire d'Analyse des Résidus en Traces - CART ; CRPP
Région wallonne : Direction générale des Technologies, de la Recherche et de l'Energie - DGTRE
I-MALDI-MS: Reconnaissance moléculaire d'agents microbiens par immuno-MALDI-MS
Researchers
http://hdl.handle.net/2268/77223

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