ORBi: Ruelle Virginie - Detection of biomarkers of pathogenic bacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Reference : Detection of biomarkers of pathogenic bacteria by matrix-assisted laser desorption/ioniz...


	Document type :	Parts of books : Contribution to collective works
	Discipline(s) :	Physical, chemical, mathematical & earth Sciences : Chemistry Life sciences : Environmental sciences & ecology
	To cite this reference:	http://hdl.handle.net/2268/77223

Title :	Detection of biomarkers of pathogenic bacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
Language :	English
Author, co-author :	Ruelle, Virginie [ > > ]
	El Moualij, B. [ > > ]
	Zorzi, Willy [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine - Département des sciences biomédicales et précliniques >]
	Zorzi, Danièle [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques >]
	Ledent, P. [ > > ]
	Pierard, Olivier [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
	Bonjean, Nicolas [ > > ]
	Gillet, Marie-Claire [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie - Cytologie - Département des sciences biomédicales et précliniques >]
	Heinen, Ernst [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
	De Pauw, Edwin [Université de Liège - ULg > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.) >]
Publication date :	2005
Main document title :	Environmental Chemistry : Green Chemistry and Pollutants in Ecosystems
Editor :	Lichtfouse, Eric
	Schwarzbauer, Jan
	Robert, Didier
Publisher :	Springer
Pages :	31-42
City :	Berlin
Country :	Germany
Keywords :	[en] MALDI-TOF MS ; bacteria ; biomarker ; activated sludge ; wastewater treatment ; optimization
Abstract :	[en] In recent years, various mass spectrometry procedures has been developed for identifying bacteria. The accuracy and speed with which data can be obtained by Matrix-assisted laser Desorption/Ionization Time-of-flight Mass Spectrometry (MALDI-TOF-MS) make this an advantageous technique for environmental monitoring. However, minor variations in the sample preparation can influence the mass spectra significantly. In the present study, we have introduced a procedure to prepare bacteria by microextraction and we have optimized experimental parameters for rapid identification by MALDI-TOF-MS of whole bacterial cells isolated from environmental samples such as wastewater and soil.
Research units :	Centre Interfacultaire d'Analyse des Résidus en Traces - CART ; CRPP
Funders :	Région wallonne : Direction générale des Technologies, de la Recherche et de l'Energie - DGTRE
Name of the research project :	I-MALDI-MS: Reconnaissance moléculaire d'agents microbiens par immuno-MALDI-MS
Target :	Researchers
Permalink :	http://hdl.handle.net/2268/77223

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