Reference : The Efficiency of In vitro Ovine Embryo Production Using an Undefined or a Defined Matur...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/77183
The Efficiency of In vitro Ovine Embryo Production Using an Undefined or a Defined Maturation Medium is Determined by the Source of the Oocyte.
English
Cocero, M. [Instituto Nacional de Investigacion y Tecnologıa Agraria y Alimentaria (INIA), Madrid, Spain > > > > > >]
Alabart, J. [Centro de Investigacion y Tecnologia Agroalimentaria (CITA) de Aragon, Zaragoza, Spain > > > > > >]
Hammami, S. [Instituto Nacional de Investigacion y Tecnologıa Agraria y Alimentaria (INIA), Madrid, Spain > > > >]
Marti, J. [Centro de Investigacion y Tecnologia Agroalimentaria (CITA) de Aragon, Zaragoza, Spain > > > > > >]
Lahoz, B. [Centro de Investigacion y Tecnologia Agroalimentaria (CITA) de Aragon, Zaragoza, Spain > > > > > >]
Sanchez, P. [Centro de Investigacion y Tecnologia Agroalimentaria (CITA) de Aragon, Zaragoza, Spain > > > > > >]
Echegoyen, E. [Centro de Investigacion y Tecnologia Agroalimentaria (CITA) de Aragon, Zaragoza, Spain > > > > > >]
Beckers, Jean-François mailto [Université de Liège - ULg > Département de sciences fonctionnelles > Physiologie de la reproduction >]
Folch, J. [Centro de Investigacion y Tecnologia Agroalimentaria (CITA) de Aragon, Zaragoza, Spain > > > > > >]
2011
Reproduction in Domestic Animals
Blackwell Publishing
46
3
463-470
Yes (verified by ORBi)
International
0936-6768
1439-0531
Berlin
Germany
[en] Embryo ; Oocyte ; Ovine ; Defined Maturation Medium
[en] In vitro oocyte maturation can be influenced by oocyte source and maturation media composition. The aim of the present study was to compare the efficiency of a defined in vitro maturation medium (TCM199 supplemented with cysteamine and epidermal growth factor; Cys + EGF) with an undefined medium (TCM199 supplemented with follicle-stimulating hormone and follicular fluid; FSH + FF) for in vitro production (IVP) of ovine embryos, using oocytes obtained by laparoscopic ovum pick-up from FSH-stimulated [n = 11; 158 cumulus-oocyte complexes (COCs)] and non-stimulated (n = 16; 120 COCs) live ewes, as well as abattoir-derived oocytes (170 COCs). The produced blastocysts were vitrified and some of them were transferred to synchronized recipients. The best and the worst final yields of embryo IVP observed in this study were obtained using oocytes from FSH-stimulated ewes matured in FSH + FF (41.3%; 33/80) and in Cys + EGF (19.2%; 15/78) medium, respectively (p < 0.01). No significant differences between both media were attained in the blastocyst development rate or in the final yield of embryo IVP using oocytes from non-stimulated ewes or abattoir-derived oocytes. The overall in vivo survival rate of the transferred vitrified blastocysts was 13.1% (8/61), without significant differences between oocyte sources or maturation media. In conclusion, under the experimental conditions of the present study, TCM199 supplemented with cysteamine and EGF is a convenient defined maturation medium for IVP of embryos from oocytes of live non-stimulated ewes or from oocytes of abattoir-derived ovaries. However, the best final yield of embryo IVP observed in this study was attained when oocytes came from FSH-stimulated donors and TCM199 was supplemented with FSH and follicular fluid
This work was supported by M° de Educacion y Ciencia (Project AGL2005-08277) and IAMZ-CIHEAM (grant for S. Hammami)
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/77183
10.1111/j.1439-0531.2010.01690.x

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