Reference : Impact of successive freezing-thawing cycles on 3-T magnetic resonance images of the dig...
Scientific journals : Article
Life sciences : Veterinary medicine & animal health
http://hdl.handle.net/2268/77172
Impact of successive freezing-thawing cycles on 3-T magnetic resonance images of the digits of isolated equine limbs
English
Bolen, Géraldine mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Département clinique des animaux de compagnie et des équidés >]
Haye, Dimitri [> >]
Dondelinger, Robert mailto [Université de Liège - ULg > Département des sciences cliniques > Département des sciences cliniques >]
Massart, Laurent [> >]
Busoni, Valeria mailto [Université de Liège - ULg > Département clinique des animaux de compagnie et des équidés > Imagerie médicale >]
Jun-2011
American Journal of Veterinary Research
American Veterinary Medical Association
72
6
780-790
Yes (verified by ORBi)
International
0002-9645
Schaumburg
IL
[en] MRI ; horses ; foot ; preservation
[en] The purpose of this study was to assess the impact of freezing and thawing on MR images of equine feet examined ex vivo. Nine equine cadaver digits were first imaged at room temperature (T0). Among the 9 digits, 3 (group 1) were imaged in a 3 Tesla MR system after one and after 2 freezing-thawing cycles. Digits of group 1 were thawed in a cold room at 4°C for 36h. Three other digits (group 2) were imaged after one freezing-thawing cycle. Digits of group 2 were thawed in a cold room at 4°C and then rescanned after 24h at room temperature. The last 3 digits (group 3) were scanned after one freezing-thawing cycle. Digits of group 3 were thawed at room temperature for 24h. Sequences used were Spin Echo (SE) T1, Turbo Spin Echo (TSE) T2 and proton density (PD), Short Tau Inversion Recovery (STIR), Double Echo Steady State (DESS), 3D Gradient Echo (GE) T1 and 2D GE T2*. Images obtained on the fresh limbs at room temperature were subjectively compared side by side to images obtained at the different freezing-thawing cycles. A quantitative analysis to assess signal change between examinations was realized by measuring signal to noise ratio (SNR). Visibility and margination of the anatomical structures of the foot and overall image quality were subjectively considered unchanged except for the hoof where the lamina was considered less visible distally after freezing and thawing in the GE T2* and in TSE T2 and PD sequences. Quantitative analysis demonstrated SNR changes in the bone marrow only in the distal phalanx in the SE T1 sequence when the feet were thawed at room temperature. When the feet were thawed in a cold room at 4°C, bone marrow SNR changes were present in the SE T1, GE T1 and TSE PD sequences. Signal changes were significant in the synovial recess when the thawing process was made at 4°C and not when the thawing process was at ambient temperature. The soft tissue structures and the hoof capsule showed significant changes with an increase of SNR, except in STIR, after freezing and thawing at 4°C and at room temperature. SNR changes in the soft tissues were mainly present in GE sequences.
Professionals ; Students
http://hdl.handle.net/2268/77172
10.2460/ajvr.72.6.780

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