Article (Scientific journals)
Immunogold Labelling of Fatty Acyl Chains
Compère, Philippe; Maneta-Peyret, Lilly; Goffinet, Gerhard et al.
1995In Journal of Immunological Methods, 181 (2), p. 201-9
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Keywords :
Immunolabelling; lipids; fatty acids
Abstract :
[en] For the first time, antibodies against a hydrophobic hapten have been used for immunogold labelling of a lipid antigen (BSA-C18:1 conjugate) coated on polystyrene. The labelling was visualised either directly in transmission electron microscopy or in light microscopy after silver enhancement. Good recognition of the fatty acyl chain was obtained even after treatment of the antigen coat with various cross-linking fixatives used for electron microscopy, i.e. formaldehyde, glutaraldehyde and osmium tetroxide.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Compère, Philippe ;  Université de Liège - ULiège > Département des sciences et gestion de l'environnement > Département des sciences et gestion de l'environnement
Maneta-Peyret, Lilly
Goffinet, Gerhard ;  Université de Liège - ULiège > Services généraux (Faculté des sciences) > Relations académiques et scientifiques (Sciences)
Cassagne, Claude
Language :
English
Title :
Immunogold Labelling of Fatty Acyl Chains
Publication date :
26 April 1995
Journal title :
Journal of Immunological Methods
ISSN :
0022-1759
eISSN :
1872-7905
Publisher :
Elsevier Science
Volume :
181
Issue :
2
Pages :
201-9
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 24 November 2010

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