Article (Scientific journals)
Species identification, molecular sexing and genotyping using non-invasive approaches in two wild bovids species: Bos gaurus and Bos javanicus.
Riviere-Dobigny, Taiana; Doan, Lan Pham; Quang, Nam Le et al.
2009In Zoo Biology, 28 (2), p. 127-36
Peer reviewed
 

Files


Full Text
Zoo Biology Rivière.pdf
Publisher postprint (360.3 kB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Animals; Animals, Zoo; Base Sequence; DNA/genetics; Feces/chemistry; Female; Hair/chemistry; Male; Molecular Sequence Data; Polymerase Chain Reaction/methods/veterinary; Ruminants/blood/classification/genetics/physiology; Sex Determination (Genetics)
Abstract :
[en] Since the second Indochina war, habitat destruction and overhunting has resulted in fragmentation of the remaining populations of Bos javanicus and B. gaurus. Nowadays, both species are in serious danger, especially the gaur. In Vietnam, where these species have become almost impossible to capture in the wild, non-invasive investigations are the only feasible approach to obtain data on populations. However, non-invasive derived DNA, especially in tropical areas, is usually characterized by low concentrations, poor quality and/or contamination from alien DNA. To assist in tropical conservation management, baseline information is provided here on assessing the reliability of species identification, molecular sexing and microsatellite genotyping using fecal DNA from B. gaurus and B. javanicus. For species identification using bovine fecal samples, cytochrome b fragment between positions 867 and 1140 was found to contain species diagnostic sites, which distinguishes the four species encountered in the region: B. gaurus, B. indicus, B. javanicus and B. taurus. For sex determination, primers were initially tested on DNA obtained from blood. Then, these primers were successfully used on DNA derived from fecal material. Finally, we also evaluate the feasibility of non-invasive microsatellite genotyping on fecal samples collected in Vietnamese nature reserves. The results presented here improve on current molecular methods based on fecal material obtained from tropical areas.
Disciplines :
Zoology
Author, co-author :
Riviere-Dobigny, Taiana
Doan, Lan Pham
Quang, Nam Le
Maillard, Jean-Charles
Michaux, Johan  ;  Université de Liège - ULiège > Département des sciences de la vie > Génétique
Language :
English
Title :
Species identification, molecular sexing and genotyping using non-invasive approaches in two wild bovids species: Bos gaurus and Bos javanicus.
Publication date :
2009
Journal title :
Zoo Biology
ISSN :
0733-3188
eISSN :
1098-2361
Publisher :
John Wiley & Sons, Hoboken, United States - New Jersey
Volume :
28
Issue :
2
Pages :
127-36
Peer reviewed :
Peer reviewed
Available on ORBi :
since 24 November 2010

Statistics


Number of views
103 (5 by ULiège)
Number of downloads
745 (3 by ULiège)

Scopus citations®
 
11
Scopus citations®
without self-citations
11
OpenCitations
 
10

Bibliography


Similar publications



Contact ORBi