A Specific Pcr to Differentiate between Ge Negative Vaccine and Wildtype Bovine Herpesvirus Type 1 Strains

[en] In the context of infectious bovine rhinotracheitis (IBR) control programmes using glycoprotein E (gE) deleted marker vaccines, a PCR assay was developed to allow the genotypic differentiation between wildtype bovine herpesvirus type 1 (BoHV-1) and gE negative strains. This assay is based on the PCR amplification of a 281 bp DNA fragment within the gE gene. The specificity of the amplification was confirmed by restriction endonuclease analysis and nucleotide sequencing of the PCR product. Its ability to determine the gE genotype of BoHV-1 strains was demonstrated on isolates coming from 20 experimental calves infected with four different BoHV-1 strains. This PCR assay may be a useful tool for monitoring the spread of live marker vaccine and the gE genotype of viral field isolates.

Disciplines :

Microbiology
Veterinary medicine & animal health

Author, co-author :

Schynts, F.

Baranowski, E.

Lemaire, Mylène; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Virologie, épidémiologie et pathologie des maladies virales

Thiry, Etienne

Language :

English

Title :

A Specific Pcr to Differentiate between Ge Negative Vaccine and Wildtype Bovine Herpesvirus Type 1 Strains

Publication date :

19 April 1999

Journal title :

Veterinary Microbiology

ISSN :

0378-1135

eISSN :

1873-2542

Publisher :

Elsevier, Netherlands

Volume :

Issue :

Pages :

187-95

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 04 March 2009

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Bibliography

Ashbaugh S.E., Thompson K.E., Belknap E.B., Schultheiss P.C., Chowdhury S., Collins J.K. Specific detection of shedding and latency of bovine herpesvirus 1 and 5 using a nested polymerase chain reaction. J. Vet. Diagn. Invest. 9:1997;387-394.
Brake F., Studdert M. Molecular epidemiology and pathogenesis of ruminant herpesviruses including bovine, buffalo and caprine herpesviruses 1 and bovine encephalitis herpesvirus. Aust. Vet. J. 62:1985;331-334.
Dangler C.A., Henderson L.M., Bowman L.A., Deaver R.E. Direct isolation and identification of recombinant pseudorabies virus strains from tissues of experimentally co-infected swine. Am. J. Vet. Res. 54:1993;540-545.
Ek-Kommonen C., Pelkonen S., Nettleton P.F. Isolation of a herpesvirus serologically related to bovine herpesvirus 1 from a reindeer (Rangifer tarandus). Acta Vet. Scand. 27:1986;299-301.
Galeota J.A., Flores E.F., Kit S., Kit M., Osorio F.A. A quantitative study of the efficacy of a deletion mutant bovine herpesvirus-1 differential vaccine in reducing the establishment of latency by wildtype virus. Vaccine. 15:1997;123-128.
Glazenburg K.L., Moormann R.J., Kimman T.G., Gielkens A.L., Peeters B.P. In vivo recombination of pseudorabies virus strains in mice. Virus Res. 34:1994;115-126.
Henderson L.M., Katz J.B., Erickson G.A., Mayfield J.E. In vivo and in vitro genetic recombination between conventional and gene-deleted vaccine strains of pseudorabies virus. Am. J. Vet. Res. 51:1990;1656-1662.
Henderson L.M., Levings R.L., Davis A.J., Sturtz D.R. Recombination of pseudorabies virus vaccine strains in swine. Am. J. Vet. Res. 52:1991;820-825.
Inglis D.M., Bowie J.M., Allan M.J., Nettleton P.F. Ocular disease in red deer calves associated with a herpesvirus infection. Vet. Rec. 113:1983;182-183.
Jacobs L. Glycoprotein E of pseudorabies virus and homologous proteins in other alphaherpesvirinae. Arch. Virol. 137:1994;209-228.
Javier R.T., Sederati F., Stevens J.G. Two avirulent herpes simplex viruses generate lethal recombinants in vivo. Science. 234:1986;746-748.
Kaashoek M.J., Moerman A., Madic J., Rijsewijk F.A., Quak J., Gielkens A.L., Van Oirschot J.T. A conventionally attenuated glycoprotein E-negative strain of bovine herpesvirus type 1 is an efficacious and safe vaccine. Vaccine. 12:1994;439-444.
Kaashoek, M.J., Rijsewijk, F.A.M., Maris-Veldhuis, M.A., Van Oirschot, J.T., 1996. Non essential glycoproteins gC, gG, gI and gE are expressed in all of 223 isolates of bovine herpesvirus. In: Kaashoek, M.J. (Ed.), Marker Vaccines Against Bovine Herpesvirus Type 1, Ph.D. Thesis. Utrecht University.
Kibenge F.S.B., Harris L.M., McKenna P.K., Wadowska D., Yason C.V. Amplification of strains of bovine herpesvirus 1 by use of polymerase chain reaction with primers in the thymidine kinase region. Am. J. Vet. Res. 55:1994;1206-1212.
Kramps J.A., Magdalena J., Quak J., Weerdmeester K., Kaashoek M.J., Maris-Veldhuis M.A., Rijsewijk F.A.M., Keil G., Van Oirschot J.T. A simple, specific, and highly sensitive blocking enzyme-linked immunosorbent assay for detection of antibodies to bovine herpesvirus 1. J. Clin. Microbiol. 32:1994;2175-2181.
Lemaire M., Schynts F., Meyer G., Thiry E. Antibody response to glycoprotein E after bovine herpesvirus type 1 infection in maternally immunised gE-negative calves. Vet. Rec. 144:1999;172-176.
Leung-Tack P., Audonnet J.C., Rivière M. The complete DNA sequence and the genetic organisation of the short unique region (US) of the bovine herpesvirus type 1 (ST strain). Virology. 119:1994;409-421.
Lyaku J.R.S., Nettleton P.F., Marsden H.S. A comparision of serological relationships among ruminant alphaherpesviruses by ELISA. Arch. Virol. 124:1992;333-341.
Lyaku J.R.S., Vilcek S., Nettleton P.F., Marsden H.S. The distinction of serologically related ruminant alphaherpesviruses by the polymerase chain reaction (PCR) and restriction endonuclease analysis. Vet. Microbiol. 48:1996;135-142.
Masri S.A., Olson W., Nguyen P.T., Prins S., Deregt D. Rapid detection of bovine herpesvirus 1 in the semen of infected bulls by a nested polymerase chain reaction assay. Can. J. Vet. Res. 60:1996;100-107.
Rebordosa X., Pinol J., Perez-Pons J.A., Lloberas J., Naval J., Querol E. Mapping, cloning and sequencing of a glycoprotein-encoding gene from bovine herpesvirus type 1 homologous to the gE gene from HSV-1. Gene. 149:1994;203-209.
Sanger F., Nicklen S., Coulson A.R. DNA sequencing with chain terminating inhibitors. Proc. Natl. Acad. Sci. USA. 74:1977;5463-5467.
Santurde G., Da Silva N., Villares R., Tabares E., Solana A., Bautista J.M., Castro J.M. Rapid and high sensitivity test for the detection of bovine herpesvirus-1 genome in clinical samples. Vet. Microbiol. 49:1996;81-92.
Schynts F., Lemaire M., Baranowski E., Thiry E. La glycoprotéine gE de l'herpesvirus bovin de type 1 et les nouveaux vaccins marqués. Ann. Méd. Vét. 142:1998;21-32.
Straub, O.C., 1996. Experience with two BHV-1 marker vaccines. In: Proc. 19th Buiatrics Congress, 1, pp. 39-41.
Strube W., Auer S., Block W., Heinen E., Kretzdorn D., Rodenbach C., Schmeer N. A gE deleted infectious bovine rhinotracheitis marker vaccine for use in improved bovine herpesvirus 1 control programs. Vet. Microbiol. 53:1996;181-189.
Szilagyi J.F., Cunningham C. Identification and characterization of a novel non-infectious herpes simplex virus-related particle. J. Gen. Virol. 72:1991;661-668.
Van Engelenburg F.A.C., Maes R.K., Van Oirschot J.T., Rijsewijk F.A.M. Development of a rapid and sensitive polymerase chain reaction assay for detection of bovine herpesvirus type 1 in bovine semen. J. Clin. Microbiol. 31:1993;3129-3135.
Van Engelenburg F.A., Kaashoek M.J., Van Oirschot J.T., Rijsewijk F.A. A glycoprotein E deletion mutant of bovine herpesvirus 1 infects the same limited number of tissues in calves as wild-type virus, but for a shorter period. J. Gen. Virol. 76:1995;2387-2392.
Van Oirschot J.T., Kaashoek M.J., Maris-Veldhuis M.A., Weerdmeester K., Rijsewijk F.A.M. An enzyme linked immunosorbent assay to detect antibodies against glycoprotein gE of bovine herpesvirus 1 allows differentiation between infected and vaccinated cattle. J. Virol. Methods. 67:1997;23-34.
Vilcek S. Detection of the bovine herpesvirus-1 (BHV-1) genome by PCR. J. Virol. Methods. 41:1993;245-248.
Wagter L.H.A., Glas R.D., Bleumink-Pluym N., Van Engelenburg F.A.C., Rijsewijk F.A.M., Houwers D.J. A polymerase chain reaction (PCR) for the detection of bovine herpesvirus 1 (BHV-1) in selectively digested whole bovine semen. Vet. Res. Commun. 20:1996;401-408.
Wiedmann M., Brandon R., Wagner P., Dubovi E.J., Batt C.A. Detection of bovine herpesvirus-1 in bovine semen by a nested PCR assay. J. Virol. Methods. 44:1993;129-140.