Article (Scientific journals)
Identification and Characterization of Bovine Herpesvirus Type 5 Glycoprotein H Gene and Gene Products
Meyer, Gilles; Bare, O.; Thiry, Etienne
1999In Journal of General Virology, 80 (Pt 11), p. 2849-59
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Abstract :
[en] Bovine herpesvirus type 5 (BHV-5) is the causative agent of a fatal meningo-encephalitis in calves and is closely related to BHV-1 which causes infectious bovine rhinotracheitis. The gene encoding BHV-5 glycoprotein gH was sequenced. A high degree of conservation was found between BHV-1 and BHV-5 deduced gH amino acid sequences (86. 4%), which is also observed for all alphaherpesvirus gH sequences. Transcriptional analysis revealed a 3.1 kb mRNA as the specific gH transcript which was detected 2 h post-infection (p.i.). Twelve out of twenty-one MAbs directed against BHV-1 gH immunoprecipitated a 108-110 kDa glycoprotein, which was then designated BHV-5 gH. Synthesis and intracellular processing of BHV- 5 gH was analysed in infected MDBK cells using gH cross-reacting MAbs. Glycoprotein gH was expressed as a beta-gamma protein, detected by radioimmunoprecipitation as early as 3 h p.i. Glycosylation studies indicated that BHV-5 gH contains N-linked carbohydrates which are essential for the recognition of the protein by the MAbs. This suggests that N-linked glycans are involved in protein folding or are targets for the gH cross-reacting MAbs. Plaque- reduction neutralization assays showed that at least one BHV-1 gH antigenic domain is lacking in BHV-5 which may possibly relate to in vivo differences in virus tropism.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Meyer, Gilles
Bare, O.;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Virologie, épidémiologie et pathologie des maladies virales
Thiry, Etienne 
Language :
English
Title :
Identification and Characterization of Bovine Herpesvirus Type 5 Glycoprotein H Gene and Gene Products
Publication date :
November 1999
Journal title :
Journal of General Virology
ISSN :
0022-1317
eISSN :
1465-2099
Publisher :
Society for General Microbiology, United Kingdom
Volume :
80
Issue :
Pt 11
Pages :
2849-59
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 04 March 2009

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