Article (Scientific journals)
Quantification by PIXE of metallic sites in proteins separated by electrophoresis
Strivay, David; Schoefs, B.; Weber, Georges
1998In Nuclear Instruments and Methods in Physics Research. Section B, Beam Interactions with Materials and Atoms, 137, p. 932-935
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Keywords :
particle induced x-ray emission; electrophoresis; protein; metal
Abstract :
[en] Electrophoresis on polyacrylamide gel (PAGE) is widely used in life sciences to determine the molecular weight of proteins in solution by separating them into different bands. By coupling electrophoresis and Particle Induced W-ray Emission (PIXE), the nature and the quantity of metals contained in proteins can be investigated. After the electrophoresis, the gel is dried and each track is scanned with a 2.5 MeV proton beam which induces X-ray emission. Analysis of these spectra allows the determination of the metals contained in an electrophoretic band. The metal content in each band is obtained by comparing the characteristic X-ray peak area with those obtained with polyacrylamide gels doped with the same metal. Finally, the relative concentration of each protein is determined by densitometry in order to compute the protein/metal ratio. An example of metallic site determination is presented. This procedure seems to be a very useful multielementary method for the determination of the metal amounts inside proteins after their separation by electrophoresis. Furthermore it allows to check if metals remain bound to proteins. (C) 1998 Elsevier Science B.V.
Disciplines :
Biochemistry, biophysics & molecular biology
Physics
Author, co-author :
Strivay, David  ;  Université de Liège - ULiège > Département de physique > Physique nucléaire, atomique et spectroscopie - Centre européen en archéométrie
Schoefs, B.
Weber, Georges ;  Université de Liège - ULiège > Centre européen en archéométrie - Physique nucléaire, atomique et spectroscopie
Language :
English
Title :
Quantification by PIXE of metallic sites in proteins separated by electrophoresis
Publication date :
1998
Journal title :
Nuclear Instruments and Methods in Physics Research. Section B, Beam Interactions with Materials and Atoms
ISSN :
0168-583X
eISSN :
1872-9584
Publisher :
Elsevier Science, Amsterdam, Netherlands
Volume :
137
Pages :
932-935
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 23 November 2010

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