Reference : Varicella-zoster virus gene 63 encodes an immediate-early protein that is abundantly exp...
Scientific journals : Article
Human health sciences : Immunology & infectious disease
http://hdl.handle.net/2268/7663
Varicella-zoster virus gene 63 encodes an immediate-early protein that is abundantly expressed during latency
English
Debrus, Serge [ > > ]
Sadzot-Delvaux, Catherine mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Virologie et immunologie - GIGA-M : Coordination scientifique >]
Nikkels, Arjen mailto [Université de Liège - ULg > > Dermatologie >]
Piette, Jacques mailto [Université de Liège - ULg > Département des sciences de la vie > GIGA-R : Virologie - Immunologie - Département des sciences de la vie - GIGA-Research >]
Rentier, Bernard mailto [Université de Liège - ULg > Services administratifs généraux > Recteur - GIGA-R : Virologie - Immunologie >]
1995
Journal of Virology
American Society of Microbiology
69
5
3240-3245
Yes (verified by ORBi)
International
0022-538X
[en] Varicella-zoster virus (VZV) gene 63 encodes a protein with a predicted molecular mass of 30.5 kDa which has amino acid similarities with the immediate-early (IE) protein 22 (ICP-22) of herpes simplex virus type 1. In order to study the expression of this protein during lytic and latent infection, gene 63 was cloned in frame and downstream from the glutathione-S-transferase gene, expressed as a fusion protein, and purified. In VZV-infected Vero cells, antibodies directed against this protein detect two polypeptides of 45 and 38 kDa which are localized both in the cytoplasm and in the nucleus. Using a sequential combination of transcription and protein synthesis inhibitors (actinomycin D and cycloheximide, respectively), we demonstrated the immediate-early nature of this protein, which can thus be named IE63. Using a rat model of VZV latency, we showed that IE63 is heavily expressed, essentially in neurons, during latency. IE63 can also be detected in the skin of patients showing early herpes zoster symptoms.
Researchers
http://hdl.handle.net/2268/7663

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