Reference : Possible Influence of Surfactants and Proteins on the Efficiency of Contact Agar Micr...
Scientific journals : Article
Life sciences : Food science
http://hdl.handle.net/2268/76440
Possible Influence of Surfactants and Proteins on the Efficiency of Contact Agar Microbiological Surface Sampling
English
Deckers, Sylvie M. [Katholieke Universiteit Leuven, Faculty of Bioscience Engineering, Kasteelpark Arenberg, 33 P.O. Box 2463, BE-3001 Heverlee, Belgium > Department of Microbial and Molecular Systems (M2S) > > >]
Sindic, Marianne mailto [Université de Liège - ULg > Chimie et bio-industries > Technologie des industries agro-alimentaires >]
Anceau, Christine [Université de Liège - ULg > Chimie et bio-industries > Technologie des industries agro-alimentaires >]
Brostaux, Yves mailto [Université de Liège - ULg > Sciences agronomiques > Statistique, Inform. et Mathém. appliquée à la bioingénierie >]
Detry, Jean G. [ > > ]
Nov-2010
Journal of Food Protection
International Association for Food Protection
73
11
2116-2122
Yes (verified by ORBi)
International
0362-028X
Des Moines
IA
[en] RODAC plates ; Petrifilm (R) ; surface sampling ; hygiene control ; bacterial recovery
[en] Agar contact microbiological sampling techniques, based on a transfer of the microorganisms present on a surface to a culture medium, are widely used to assess and control surface cleanliness and to evaluate microbial contamination levels. The effectiveness of these techniques depends on many environmental parameters that influence the strength of attachment of the bacteria to the surface. In the present study, stainless steel and high density polyethylene surfaces were inoculated with known concentrations of Staphylococcus epidermidis. Following an experimental design, the surfaces were sampled with different types of replicate organism direct agar contact plates and Petrifilm; results indicated that recovery rates were influenced by the presence of egg white albumin or Tween 80 in the inoculum solutions or by the introduction of surfactants into the contact agar of the microbiological sampling techniques. The techniques yielded significantly different results, depending on sampling conditions, underlining the need for a standardization of laboratory experiments to allow relevant comparisons of such techniques.
Walloon Region (DGTRE and DGA) ; 3M Belgium and LED Techno N.V./S.A.
http://hdl.handle.net/2268/76440

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