Article (Scientific journals)
Possible Influence of Surfactants and Proteins on the Efficiency of Contact Agar Microbiological Surface Sampling
Deckers, Sylvie M.; Sindic, Marianne; Anceau, Christine et al.
2010In Journal of Food Protection, 73 (11), p. 2116-2122
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Keywords :
RODAC plates; Petrifilm (R); surface sampling; hygiene control; bacterial recovery
Abstract :
[en] Agar contact microbiological sampling techniques, based on a transfer of the microorganisms present on a surface to a culture medium, are widely used to assess and control surface cleanliness and to evaluate microbial contamination levels. The effectiveness of these techniques depends on many environmental parameters that influence the strength of attachment of the bacteria to the surface. In the present study, stainless steel and high density polyethylene surfaces were inoculated with known concentrations of Staphylococcus epidermidis. Following an experimental design, the surfaces were sampled with different types of replicate organism direct agar contact plates and Petrifilm; results indicated that recovery rates were influenced by the presence of egg white albumin or Tween 80 in the inoculum solutions or by the introduction of surfactants into the contact agar of the microbiological sampling techniques. The techniques yielded significantly different results, depending on sampling conditions, underlining the need for a standardization of laboratory experiments to allow relevant comparisons of such techniques.
Disciplines :
Food science
Author, co-author :
Deckers, Sylvie M.;  Katholieke Universiteit Leuven, Faculty of Bioscience Engineering, Kasteelpark Arenberg, 33 P.O. Box 2463, BE-3001 Heverlee, Belgium > Department of Microbial and Molecular Systems (M2S)
Sindic, Marianne  ;  Université de Liège - ULiège > Chimie et bio-industries > Technologie des industries agro-alimentaires
Anceau, Christine ;  Université de Liège - ULiège > Chimie et bio-industries > Technologie des industries agro-alimentaires
Brostaux, Yves  ;  Université de Liège - ULiège > Sciences agronomiques > Statistique, Inform. et Mathém. appliquée à la bioingénierie
Detry, Jean G.
Language :
English
Title :
Possible Influence of Surfactants and Proteins on the Efficiency of Contact Agar Microbiological Surface Sampling
Publication date :
November 2010
Journal title :
Journal of Food Protection
ISSN :
0362-028X
eISSN :
1944-9097
Publisher :
International Association for Food Protection, Des Moines, United States - Iowa
Volume :
73
Issue :
11
Pages :
2116-2122
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
Région wallonne [BE]
3M Belgium
LED Techno N.V./S.A.
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