Reference : Improved antigenic methods for differential diagnosis of bovine, caprine, and cervine al...
Scientific journals : Article
Life sciences : Microbiology
http://hdl.handle.net/2268/7632
Improved antigenic methods for differential diagnosis of bovine, caprine, and cervine alphaherpesviruses related to bovine herpesvirus 1
English
Keuser, V. [> > > >]
Schynts, F. [> > > >]
Detry, Bruno [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
Mathieu, Anne mailto [> > > >]
Robert, B. [Université de Liège - ULg > > Immunologie et vaccinologie >]
Vanderplasschen, Alain mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Département des maladies infectieuses et parasitaires >]
Pastoret, Paul-Pierre mailto [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Virologie, épidémiologie et pathologie des maladies virales >]
Thiry, Etienne mailto [> > > >]
Mar-2004
Journal of Clinical Microbiology
Amer Soc Microbiology
42
3
1228-1235
Yes (verified by ORBi)
International
0095-1137
Washington
[en] The control of infectious bovine rhinotracheitis induced by bovine herpesvirus 1 (BoHV-1) requires sensitive and specific diagnostic assays. As BoHV-1 is antigenically and genetically related to four other alphaherpesviruses of ruminants-namely, BoHV-5, caprine herpesvirus 1 (CpHV-1), cervine herpesvirus 1 (CvHV-1) and CvHV-2-diagnostic tests able to discriminate BoHV-1 from these related viruses are needed to avoid misdiagnosis, especially because some of these viruses are able to cross the species barrier. In this study, murine monoclonal antibodies (MAbs) specific for BoHV-1, BoHV-5, CpHV-1, CvHV-1, and CvHV-2 were produced with the aim of setting up an immunofluorescence assay able to discriminate between these related herpesviruses. Produced MAbs were selected for their viral specificity by enzyme-linked immunosorbent assay and indirect immunofluorescence staining of virus-infected cells. Radioimmunoprecipitation characterization of the selected MAbs revealed that four of them are directed against glycoprotein C (gC) and one of them is directed against gD of these related viruses. The obtained results demonstrate that the antibodies produced allow an unambiguous discrimination of each of the four alphaherpesviruses related to BoHV-1.
http://hdl.handle.net/2268/7632

File(s) associated to this reference

Fulltext file(s):

FileCommentaryVersionSizeAccess
Restricted access
235pub_thiry.pdfNo commentaryAuthor postprint717.81 kBRequest copy

Bookmark and Share SFX Query

All documents in ORBi are protected by a user license.