Article (Scientific journals)
Point mutations in an infectious bovine viral diarrhoea virus type 2 cDNA transcript that yields an attenuated and protective viral progeny
Dehan, Pierre; Couvreur, B.; Hamers, Claude et al.
2005In Vaccine, 23 (33), p. 4236-4246
Peer Reviewed verified by ORBi
 

Files


Full Text
252pub_thiry.pdf
Author postprint (500.69 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
pestivirus; vaccine; bovine
Abstract :
[en] An infectious cDNA clone of the hypervirulent bovine viral diarrhoea virus (BVDV) strain 890 (isolate 256) was produced by a streamlined PCR procedure. As compared to the published sequence of strain 890, the nucleotide sequencing of cloned cDNA corresponding to isolate 256 revealed several mutations seven of which were attributed to the cloning procedure. The infectious transcript was transfected into permissive cells and led to viral multiplication (AvrII+ strain). In vitro, viral titres reached by the parental strain exceed those of the AvrII+ strain by more than one order of magnitude. The latter was clearly less virulent to young calves as indicated by clinical, haematological and virological parameters. Thirty-four days after inoculation with AvrII+ strain, calves were challenged with the virulent parental strain. The animals were protected as compared to unvaccinated controls. Therefore, our approach led to the production of an attenuated strain with potential use as a vaccine strain and will be useful for studies of virulence determinants in BVDV-2. (c) 2005 Elsevier Ltd. All rights reserved.
Disciplines :
Veterinary medicine & animal health
Laboratory medicine & medical technology
Immunology & infectious disease
Author, co-author :
Dehan, Pierre ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques
Couvreur, B.
Hamers, Claude ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Immunologie et vaccinologie
Lewalle, P.
Thiry, Etienne ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Virologie, épidémiologie et pathologie des maladies virales
Kerkhofs, P.
Pastoret, Paul-Pierre ;  Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Département des maladies infectieuses et parasitaires
Language :
English
Title :
Point mutations in an infectious bovine viral diarrhoea virus type 2 cDNA transcript that yields an attenuated and protective viral progeny
Publication date :
21 July 2005
Journal title :
Vaccine
ISSN :
0264-410X
eISSN :
1873-2518
Publisher :
Elsevier Sci Ltd, Oxford, United Kingdom
Volume :
23
Issue :
33
Pages :
4236-4246
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 04 March 2009

Statistics


Number of views
72 (4 by ULiège)
Number of downloads
1 (1 by ULiège)

Scopus citations®
 
4
Scopus citations®
without self-citations
4
OpenCitations
 
4

Bibliography


Similar publications



Contact ORBi