ORBi: Dehan Pierre - Point mutations in an infectious bovine viral diarrhoea virus type 2 cDNA transcript that yields an attenuated and protective viral progeny

Reference : Point mutations in an infectious bovine viral diarrhoea virus type 2 cDNA transcript tha...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Veterinary medicine & animal health Human health sciences : Laboratory medicine & medical technology Human health sciences : Immunology & infectious disease
	To cite this reference:	http://hdl.handle.net/2268/7622

Title :	Point mutations in an infectious bovine viral diarrhoea virus type 2 cDNA transcript that yields an attenuated and protective viral progeny
Language :	English
Author, co-author :	Dehan, Pierre [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Anatomie et cytologie pathologiques >]
	Couvreur, B. [> > > >]
	Hamers, C. [> > > >]
	Lewalle, P. [> > > >]
	Thiry, Etienne [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Virologie, épidémiologie et pathologie des maladies virales >]
	Kerkhofs, P. [> > > >]
	Pastoret, Paul-Pierre [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Département des maladies infectieuses et parasitaires >]
Publication date :	21-Jul-2005
Journal title :	Vaccine
Publisher :	Elsevier Sci Ltd
Volume :	23
Issue/season :	33
Pages :	4236-4246
Peer reviewed :	Yes (verified by ORBi)
Audience :	International
ISSN :	0264-410X
City :	Oxford
Keywords :	[en] pestivirus ; vaccine ; bovine
Abstract :	[en] An infectious cDNA clone of the hypervirulent bovine viral diarrhoea virus (BVDV) strain 890 (isolate 256) was produced by a streamlined PCR procedure. As compared to the published sequence of strain 890, the nucleotide sequencing of cloned cDNA corresponding to isolate 256 revealed several mutations seven of which were attributed to the cloning procedure. The infectious transcript was transfected into permissive cells and led to viral multiplication (AvrII+ strain). In vitro, viral titres reached by the parental strain exceed those of the AvrII+ strain by more than one order of magnitude. The latter was clearly less virulent to young calves as indicated by clinical, haematological and virological parameters. Thirty-four days after inoculation with AvrII+ strain, calves were challenged with the virulent parental strain. The animals were protected as compared to unvaccinated controls. Therefore, our approach led to the production of an attenuated strain with potential use as a vaccine strain and will be useful for studies of virulence determinants in BVDV-2. (c) 2005 Elsevier Ltd. All rights reserved.
Permalink :	http://hdl.handle.net/2268/7622
DOI :	10.1016/j.vaccine.2005.03.026

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