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| Reference : Inhibition of bovine sperm-zona binding by bovine herpesvirus-1 |
| Scientific journals : Article | |||
| Life sciences : Biochemistry, biophysics & molecular biology Life sciences : Anatomy (cytology, histology, embryology...) & physiology | |||
| http://hdl.handle.net/2268/7621 | |||
| Inhibition of bovine sperm-zona binding by bovine herpesvirus-1 | |
| English | |
| Tanghe, S. [> > > >] | |
| Vanroose, G. [> > > >] | |
| Van Soom, A. [> > > >] | |
| Duchateau, L. [> > > >] | |
| Ysebaert, M. T. [> > > >] | |
| Kerkhofs, P. [> > > >] | |
Thiry, Etienne  [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Virologie, épidémiologie et pathologie des maladies virales >] | |
| Littel-van den Hurk, S. V. [> > > >] | |
| Van Oostveldt, P. [> > > >] | |
| Nauwynck, H. [> > > >] | |
| Aug-2005 | |
| Reproduction | |
| Bio Scientifica Ltd | |
| 130 | |
| 2 | |
| 251-259 | |
| International | |
| 1470-1626 | |
| Bristol | |
| [en] The purpose of the present study was to identify a potential interference of bovine herpesvirus-1 (BoHV-1) with sperm-oocyte interactions during bovine in vitro fertilization. An inhibition of almost 70% of sperm-zona binding was observed when bovine cumulus-denuded oocytes were inseminated in the presence of 10(7) 50% tissue culture infective dose/ml BoHV-1. The inhibitory effect of BoHV-1 on sperm-zona binding was mediated by an interaction of the virus with spermatozoa, but not with oocytes. Treatment of spermatozoa with BoHV-1, however, did not affect sperm motility and acrosomal status. Antiserum against RoHV-1 prevented the virus-induced inhibition of sperm-zona binding, indicating that BoHV-1 itself affects the fertilization process. In order to investigate which BoHV-1 glycoprotein(s) are responsible for the virus-sperm interaction, BoHV-1 was treated with monoclonal antibodies against the viral glycoproteins gB, gC, gD and gH prior to insemination. Anti-gC completely prevented the inhibitory effect of BoHV-1 on sperm-zona binding, while anti-gD caused a reduction of this inhibition. Further evidence for the involvement of gC and gD in the virus-sperm interaction was provided by the fact that purified gC and gD decreased sperm-zona binding in a dose-dependent way with gC being more effective than gD. These results indicated that BoHV-1 inhibits bovine sperm-zona binding by interacting with spermatozoa. The binding of BoHV-1 to a spermatozoon is mediated by the viral glycoproteins gC and gD, and therefore seems to be comparable with the mechanisms of BoHV-1 attachment to its natural host cell. | |
| http://hdl.handle.net/2268/7621 |
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