Article (Scientific journals)
Bovine herpesvirus 4 immediate early 2 (Rta) gene is an essential gene and is duplicated in bovine herpesvirus 4 isolate U.
Franceschi, V.; Capocefalo, A.; Ravanetti, L. et al.
2011In Veterinary Microbiology
Peer Reviewed verified by ORBi
 

Files


Full Text
VetMicrob2010.pdf
Publisher postprint (1.59 MB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Abstract :
[en] The ORF50/Rta gene has been shown to be an essential gene for many gammaherpesviruses. Although the BoHV-4 ORF50/Rta homolog, immediate early gene 2 (IE2), has been shown to activate several BoHV-4 early and late promoters in cotransfection assays, there is no direct proof of its indispensability for progression of the virus to the lytic replication cycle in the context of the viral genome. In the present communication, replication defective BoHV-4-V.test IE2 mutants were efficiently rescued, with respect to production of infectious virus and DNA replication, upon the expression of BoHV-4 ORF50/Rta in trans. Surprisingly, in the course of our studies, we discovered that the IE2 gene is duplicated in the genome of BoHV-4-U.
Disciplines :
Microbiology
Author, co-author :
Franceschi, V.
Capocefalo, A.
Ravanetti, L.
Vanderplasschen, Alain ;  Université de Liège - ULiège > Immunologie et vaccinologie
Gillet, Laurent  ;  Université de Liège - ULiège > Immunologie et vaccinologie
Cavirani, S.
van Santen, V. L.
Donofrio, G.
Language :
English
Title :
Bovine herpesvirus 4 immediate early 2 (Rta) gene is an essential gene and is duplicated in bovine herpesvirus 4 isolate U.
Publication date :
2011
Journal title :
Veterinary Microbiology
ISSN :
0378-1135
eISSN :
1873-2542
Publisher :
Elsevier Science, Amsterdam, Netherlands
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Copyright (c) 2010 Elsevier B.V. All rights reserved.
Available on ORBi :
since 20 November 2010

Statistics


Number of views
84 (5 by ULiège)
Number of downloads
1 (1 by ULiège)

Scopus citations®
 
9
Scopus citations®
without self-citations
2
OpenCitations
 
7

Bibliography


Similar publications



Contact ORBi