Reference : Bovine herpesvirus 4 immediate early 2 (Rta) gene is an essential gene and is duplicated...
Scientific journals : Article
Life sciences : Microbiology
http://hdl.handle.net/2268/76183
Bovine herpesvirus 4 immediate early 2 (Rta) gene is an essential gene and is duplicated in bovine herpesvirus 4 isolate U.
English
Franceschi, V. [> > > >]
Capocefalo, A. [> > > >]
Ravanetti, L. [> > > >]
Vanderplasschen, Alain mailto [Université de Liège - ULg > > Immunologie et vaccinologie >]
Gillet, Laurent mailto [Université de Liège - ULg > > Immunologie et vaccinologie >]
Cavirani, S. [> > > >]
van Santen, V. L. [> > > >]
Donofrio, G. [> > > >]
2011
Veterinary Microbiology
Elsevier Science
Yes (verified by ORBi)
International
0378-1135
Amsterdam
The Netherlands
[en] The ORF50/Rta gene has been shown to be an essential gene for many gammaherpesviruses. Although the BoHV-4 ORF50/Rta homolog, immediate early gene 2 (IE2), has been shown to activate several BoHV-4 early and late promoters in cotransfection assays, there is no direct proof of its indispensability for progression of the virus to the lytic replication cycle in the context of the viral genome. In the present communication, replication defective BoHV-4-V.test IE2 mutants were efficiently rescued, with respect to production of infectious virus and DNA replication, upon the expression of BoHV-4 ORF50/Rta in trans. Surprisingly, in the course of our studies, we discovered that the IE2 gene is duplicated in the genome of BoHV-4-U.
http://hdl.handle.net/2268/76183
10.1016/j.vetmic.2010.09.031
Copyright (c) 2010 Elsevier B.V. All rights reserved.

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