Generation of a transposon insertion mutant library for bovine herpesvirus 4 cloned as a bacterial artificial chromosome by in vitro MuA based DNA transposition system.
[en] Bovine herpesvirus 4 (BoHV-4) is a gammaherpesvirus with no clear disease association. Although the BoHV-4 genome has been sequenced, the function of the majority of putative genes is elusive. Several features make BoHV-4 attractive as a backbone for use as a viral expression vector and/or as a model to study gamma herpesvirus biology and determining which genes are essential for its replication is a very important task. Starting from BoHV-4 genome cloned as infectious bacterial artificial chromosome (BAC-BoHV-4) in Escherichia coli. A random insertion mutant library for BoHV4 was generated by the use of MuA transposase-catalyzed in vitro transposition reaction. Viral mutant transfection and direct sequencing allow the rapid determination of which BoHV-4 genes are essential for viral growth in a permissive eukaryotic cell line. BoHV-4 functional analysis information is fundamental when the BoHV-4 genome is modified for vector purposes.
Gillet, Laurent ; Université de Liège - ULiège > Immunologie et vaccinologie
Language :
English
Title :
Generation of a transposon insertion mutant library for bovine herpesvirus 4 cloned as a bacterial artificial chromosome by in vitro MuA based DNA transposition system.
Asano A., Inoshima Y., Murakami K., Iketani Y., Yamamoto Y., and Sentsui H. Latency and persistence of bovine herpesvirus type 4, strain b11-41, in bovine nervous tissues. J. Vet. Med. Sci. 65 (2003) 87-93
Ausubel F.M., Brent R., Kingston R.E., Moore D.D., Seidman J.G., Smith J.A., and Struhl K. Current Protocols in Molecular Biology vol. 1 (1989), John Wiley & Sons, New York, NY 1.8.4-1.8.10
Bartha A., Fadol A.M., Liebermann H., Ludwig H., Mohanty S.B., Osorio F.A., Reed D.E., Storz J., Straub O.C., Van der Maaten M.J., and Wellermans G. Problems concerning the taxonomy of the "Movar-Type" bovine herpesvirus. Intervirology 28 (1987) 1-7
Bartha A., Juhasz M., and Liebermann A. Isolation of a bovine herpesvirus from calves with respiratory disease and keratoconjunctivitis. Acta Vet. Acad. Sci. Hung. 16 (1966) 357-358
Bublot M., Lomonte P., Lequarre A.S., Albrecht J.C., Nicholas J., Fleckenstein B., Pastoret P.P., and Thiry E. Genetic relationships between bovine herpesvirus 4 and the gammaherpesviruses Epstein-Barr virus and herpesvirus saimiri. Virology 190 (1992) 654-665
Chaconas G., Gloor G., and Miller J.L. Amplification and purification of the bacteriophage Mu encoded B transposition protein. J. Biol. Chem. 260 (1985) 2662-2669
Chaconas G., Lavoie B.D., and Watson M.A. DNA transposition: jumping gene machine, some assembly required. Curr. Biol. 6 (1996) 817-820
Craigie R., and Mizuuchi K. Transposition of Mu DNA: joining of Mu to target DNA can be uncoupled from cleavage at the ends of Mu. Cell 51 (1987) 493-501
Donofrio G., and van Santen V.L. A bovine macrophage cell line supports bovine herpesvirus-4 persistent infection. J. Gen. Virol. 82 (2001) 1181-1185
Donofrio G., Cavirani S., Taddei S., and van Santen V.L. Potential of bovine herpesvirus 4 as a gene delivery vector. J. Virol. Methods 101 (2002) 49-61
Donofrio G., Grandi G., Cavirani S., Taddei S., and Flammini C. Bovine herpesvirus 4 infects differentiated neuronal cells in culture and establish persistent infection upon selection. J. Neurovirol. 10 (2004) 123-130
Dubuisson J., Thiry E., Bublot M., Thomas I., van Bressem M.F., Coignoul F., and Pastoret P.P. Experimental infection of bulls with a genital isolate of bovine herpesvirus-4 and reactivation of latent virus with dexamethasone. Vet. Microbiol. 21 (1989) 97-114
Egyed L., and Bartha A. PCR studies on the potential sites for latency of BHV-4 in calves. Vet. Res. Commun. 22 (1998) 209-216
Ehlers B., Buhk H.J., and Ludwig H. Analysis of bovine cytomegalovirus genome structure: cloning and mapping of the monomeric polyrepetitive DNA unit, and comparison of European and American strains. J. Gen. Virol. 66 (1985) 55-68
Gillet L., Daix V., Donofrio G., Wagner M., Koszinowski U.H., China B., Ackermann M., Markine-Goriaynoff N., and Vanderplasschen A. Development of bovine herpesvirus 4 as an expression vector using bacterial artificial chromosome cloning. J. Gen. Virol. 86 (2005) 907-917
Haapa S., Taira S., Heikkinen E., and Savilahti H. An efficient and accurate integration of mini-Mu transposons in vitro: a general methodology for functional genetic analysis and molecular biology applications. Nucleic Acids Res. 27 (1999) 2777-2784
Krogman L.A., and McAdaragh J.P. Recrudescence of bovine herpesvirus-5 in experimentally infected calves. Am. J. Vet. Res. 43 (1982) 336-338
Lopez O.J., Galeotta J.A., and Osorio F.A. Bovine herpesvirus type-4 (BHV-4) persistently infects cells of the marginal zone of spleen in cattle. Microb. Pathog. 21 (1996) 47-58
Mohanty S.B., Hammond R.C., and Lillie M.G. A new bovine herpesvirus and its effect on experimentally infected calves. Arch. Gesamte Virusforschung 34 (1971) 394-395
Naeem K., Caywood D.D., Goyal S.M., Werdin R.E., and Murtaugh M.P. Persistence of bovid herpesvirus-4 in experimentally inoculated pregnant rabbits. Microbiologica 16 (1993) 87-94
Osorio F.A., and Reed D.E. Experimental inoculation of cattle with bovine herpesvirus-4: evidence for a lymphoid-associated persistent infection. Am. J. Vet. Res. 44 (1983) 975-980
Osorio F.A., Rock D.L., and Reed D.E. Studies on the pathogenesis of a bovine cytomegalo-like virus in an experimental host. J. Gen. Virol. 66 (1985) 1941-1951
Roizman B., and Sears E. Herpes simplex viruses and their replication. In: Fields B.N., Knipe D.M., and Howley P.M. (Eds). Fundamental Virology. third ed. (1996), Lippincott-Raven, Philadelphia, PA 1043-1107
Savilahti H., Rice P.A., and Mizuuchi K. The phage Mu transpososome core: DNA requirements for assembly and function. EMBO J. 14 (1995) 4893-4903
Storz J., Ehlers B., Todd W.J., and Ludwig H. Bovine cytomegaloviruses: identification and differential properties. J. Gen. Virol. 65 (1984) 697-706
Thiry E., Bublot M., Dubuisson J., and Pastoret P.-P. Bovine herpesvirus-4 (BHV-4) infections of cattle. In: Wittmann G. (Ed). Herpesvirus Diseases of Cattle, Horses, and Pigs (1989), Kluwer Academic Publishers, Boston 96-115
Thiry E., Bublot M., Dubuisson J., Van Bressem M.F., Lequarre A.S., Lomonte P., Vanderplasschen A., and Pastoret P.P. Molecular biology of bovine herpesvirus type 4. Vet. Microbiol. 33 (1992) 79-92
Vilen H., Aalto J.M., Kassinen A., Paulin L., and Savilahti H. A direct transposon insertion tool for modification and functional analysis of viral genomes. J. Virol. 77 (2003) 123-134
Zambrowicz B.P., Immoto A., Fiering S., Herzenberg L.A., Kerr W.G., and Soriano P. Disruption of overlapping transcripts in the ROSA beta geo 26 gene trap strain leads to widespread expression of beta-galactosidase in mouse embryos and hematopoietic cells. Proc. Natl. Acad. Sci. U.S.A. 94 (1997) 3789-3794
Zimmermann W., Broll H., Ehlers B., Buhk H.J., Rosenthal A., and Goltz M. Genome sequence of bovine herpesvirus 4, a bovine Rhadinovirus, and identification of an origin of DNA replication. J. Virol. 75 (2001) 1186-1194