Reference : Glycoprotein B switches conformation during murid herpesvirus 4 entry.
Scientific journals : Article
Life sciences : Microbiology
http://hdl.handle.net/2268/76171
Glycoprotein B switches conformation during murid herpesvirus 4 entry.
English
Gillet, Laurent mailto [Université de Liège - ULg > > Immunologie et vaccinologie >]
Colaco, Susanna [> > > >]
Stevenson, Philip G [> > > >]
2008
Journal of General Virology (The)
Society for General Microbiology
89
Pt 6
1352-63
Yes (verified by ORBi)
International
0022-1317
1465-2099
London
United Kingdom
[en] Animals ; Antibodies, Viral/immunology ; Cell Line ; Cricetinae ; Cricetulus ; Glycoproteins/metabolism ; Herpesviridae Infections/virology ; Humans ; Mice ; Neutralization Tests ; Protein Conformation ; Rhadinovirus/chemistry/physiology ; Tumor Virus Infections/virology ; Viral Proteins/metabolism ; Virus Attachment
[en] Herpesviruses are ancient pathogens that infect all vertebrates. The most conserved component of their entry machinery is glycoprotein B (gB), yet how gB functions is unclear. A striking feature of the murid herpesvirus 4 (MuHV-4) gB is its resistance to neutralization. Here, we show by direct visualization of infected cells that the MuHV-4 gB changes its conformation between extracellular virions and those in late endosomes, where capsids are released. Specifically, epitopes on its N-terminal cell-binding domain become inaccessible, whilst non-N-terminal epitopes are revealed, consistent with structural changes reported for the vesicular stomatitis virus glycoprotein G. Inhibitors of endosomal acidification blocked the gB conformation switch. They also blocked capsid release and the establishment of infection, implying that the gB switch is a key step in entry. Neutralizing antibodies could only partially inhibit the switch. Their need to engage a less vulnerable, upstream form of gB, because its fusion form is revealed only in endosomes, helps to explain why gB-directed MuHV-4 neutralization is so difficult.
http://hdl.handle.net/2268/76171
10.1099/vir.0.83519-0

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