The Murid Herpesvirus-4 gL regulates an entry-associated conformation change in gH.

Animals; Cricetinae; Dimerization; Endocytosis; Endosomes/metabolism; Glycoproteins/chemistry; Hydrogen-Ion Concentration; Membrane Fusion; Membrane Glycoproteins/metabolism/physiology; Mice; Molecular Chaperones/metabolism/physiology; NIH 3T3 Cells; Protein Conformation; Rhadinovirus/metabolism; Viral Envelope Proteins/metabolism/physiology; Viral Proteins/chemistry/metabolism/physiology

Abstract :

[en] The glycoprotein H (gH)/gL heterodimer is crucial for herpesvirus membrane fusion. Yet how it functions is not well understood. The Murid Herpesvirus-4 gH, like that of other herpesviruses, adopts its normal virion conformation by associating with gL. However, gH switched back to a gL-independent conformation after virion endocytosis. This switch coincided with a conformation switch in gB and with capsid release. Virions lacking gL constitutively expressed the down-stream form of gH, prematurely switched gB to its down-stream form, and showed premature capsid release with poor infectivity. These data argue that gL plays a key role in regulating a gH and gB functional switch from cell binding to membrane fusion.

Disciplines :

Microbiology

Author, co-author :

Gillet, Laurent ; Université de Liège - ULiège > Immunologie et vaccinologie

Colaco, Susanna

Stevenson, Philip G

Language :

English

Title :

The Murid Herpesvirus-4 gL regulates an entry-associated conformation change in gH.

Publication date :

2008

Journal title :

PLoS ONE

eISSN :

1932-6203

Publisher :

Public Library of Science, San Franscisco, United States - California

Volume :

Issue :

Pages :

e2811

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 20 November 2010

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Bibliography

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