In vivo importance of heparan sulfate-binding glycoproteins for murid herpesvirus-4 infection.

Animals; Cell Line; Cricetinae; Female; Glycoproteins/genetics/metabolism; Heparitin Sulfate/metabolism; Kidney/cytology/virology; Mice; NIH 3T3 Cells; Protein Binding; Receptors, Virus/metabolism; Rhadinovirus/genetics/metabolism/pathogenicity; Viral Envelope Proteins/genetics/metabolism

Abstract :

[en] Many herpesviruses bind to heparan sulfate (HS). Murid herpesvirus-4 (MuHV-4) does so via its envelope glycoproteins gp70 and gH/gL. MuHV-4 gp150 further regulates an HS-independent interaction to make that HS-dependent too. Cell binding by MuHV-4 virions is consequently strongly HS-dependent. Gp70 and gH/gL show some in vitro redundancy: an antibody-mediated blockade of HS binding by one is well tolerated, whereas a blockade of both severely impairs infection. In order to understand the importance of HS binding for MuHV-4 in vivo, we generated mutants lacking both gL and gp70. As expected, gL(-)gp70(-) MuHV-4 showed very poor cell binding. It infected mice at high dose but not at low dose, indicating defective host entry. But once entry occurred, host colonization, which for MuHV-4 is relatively independent of the infection dose, was remarkably normal. The gL(-)gp70(-) entry deficit was much greater than that of gL(-) or gp70(-) single knockouts. And gp150 disruption, which allows HS-independent cell binding, largely rescued the gL(-)gp70(-) cell binding and host entry deficits. Thus, it appeared that MuHV-4 HS binding is important in vivo, principally for efficient host entry.

Disciplines :

Microbiology

Author, co-author :

Gillet, Laurent ; Université de Liège - ULiège > Immunologie et vaccinologie

May, Janet S

Stevenson, Philip G

Language :

English

Title :

In vivo importance of heparan sulfate-binding glycoproteins for murid herpesvirus-4 infection.

Publication date :

2009

Journal title :

Journal of General Virology

ISSN :

0022-1317

eISSN :

1465-2099

Publisher :

Society for General Microbiology, London, United Kingdom

Volume :

Issue :

Pt 3

Pages :

602-13

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 20 November 2010

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