ORBi: Muylkens Benoît - Intraspecific bovine herpesvirus 1 recombinants carrying glycoprotein E deletion as a vaccine marker are virulent in cattle

Reference : Intraspecific bovine herpesvirus 1 recombinants carrying glycoprotein E deletion as a va...


	Document type :	Scientific journals : Article
	Discipline(s) :	Life sciences : Microbiology Human health sciences : Immunology & infectious disease Life sciences : Biotechnology
	To cite this reference:	http://hdl.handle.net/2268/7608

Title :	Intraspecific bovine herpesvirus 1 recombinants carrying glycoprotein E deletion as a vaccine marker are virulent in cattle
Language :	English
Author, co-author :	Muylkens, Benoît [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Virologie, épidémiologie et pathologie des maladies virales >]
	Meurens, F. [> > > >]
	Schynts, F. [> > > >]
	Farnir, Frédéric [Université de Liège - ULg > Département de productions animales > Biostatistique, économie, sélection animale >]
	Pourchet, A. [> > > >]
	Bardiau, Marjorie [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Bactériologie et pathologie des maladies bactériennes >]
	Gogev, S. [> > > >]
	Thiry, Julien [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Virologie, épidémiologie et pathologie des maladies virales >]
	Cuisenaire, A. [> > > >]
	Vanderplasschen, Alain [Université de Liège - ULg > > Immunologie et vaccinologie >]
	Thiry, Etienne [Université de Liège - ULg > Département des maladies infectieuses et parasitaires > Virologie, épidémiologie et pathologie des maladies virales >]
Publication date :	Aug-2006
Journal title :	Journal of General Virology
Publisher :	Soc General Microbiology
Volume :	87
Issue/season :	Pt 8
Pages :	2149-2154
Audience :	International
ISSN :	0022-1317
City :	Reading
Abstract :	[en] Vaccines used in control programmes of Bovine herpesvirus 1 (BoHV-1) utilize highly attenuated BoHV-1 strains marked by a deletion of the glycoprotein E (gE) gene. Since BoHV-1 recombinants are obtained at high frequency in experimentally coinfected cattle, the consequences of recombination on the virulence of gE-negative BoHV-1 were investigated. Thus, gE-negative BoHV-1 recombinants were generated in vitro from several virulent BoHV-1 and one mutant BoHV-1 deleted in the gC and gE genes. Four gE-negative recombinants were tested in the natural host. All the recombinants were more virulent than the gE-negative BoHV-1 vaccine and the gC- and gE-negative parental BoHV-1. The gE-negative recombinant isolated from a BoHV-1 field strain induced the highest severe clinical score. Latency and reactivation studies showed that three of the recombinants were reexcreted. Recombination can therefore restore virulence of gE-negative BoHV-1 by introducing the gE deletion into a different virulence background.
Permalink :	http://hdl.handle.net/2268/7608
DOI :	10.1099/vir.0.81969-0

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