Reference : Increasing seed oil content in oil-seed rape (Brassica napus L.) by over-expression of a...
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/75855
Increasing seed oil content in oil-seed rape (Brassica napus L.) by over-expression of a yeast glycerol-3-phosphate dehydrogenase under the control of a seed-specific promoter.
English
Vigeolas, Hélène [Université de Liège - ULg > Département des sciences de la vie > Génétique >]
Waldeck, Peter [> > > >]
Zank, Thorsten [> > > >]
Geigenberger, Peter [> > > >]
2007
Plant Biotechnology Journal
Blackwell Publishing
5
3
431-41
Yes (verified by ORBi)
International
1467-7644
1467-7652
Oxford
United Kingdom
[en] Brassica napus/embryology/genetics/metabolism ; Fatty Acids/metabolism ; Glycerolphosphate Dehydrogenase/genetics/metabolism ; Glycerophosphates/metabolism ; Lipid Metabolism/genetics ; Plant Oils/metabolism ; Plants, Genetically Modified/metabolism ; Promoter Regions, Genetic ; Saccharomyces cerevisiae Proteins/genetics/metabolism ; Seeds/genetics/metabolism ; Transformation, Genetic
[en] Previous attempts to manipulate oil synthesis in plants have mainly concentrated on the genes involved in the biosynthesis and use of fatty acids, neglecting the possible role of glycerol-3-phosphate supply on the rate of triacylglycerol synthesis. In this study, a yeast gene coding for cytosolic glycerol-3-phosphate dehydrogenase (gpd1) was expressed in transgenic oil-seed rape under the control of the seed-specific napin promoter. It was found that a twofold increase in glycerol-3-phosphate dehydrogenase activity led to a three- to fourfold increase in the level of glycerol-3-phosphate in developing seeds, resulting in a 40% increase in the final lipid content of the seed, with the protein content remaining substantially unchanged. This was accompanied by a decrease in the glycolytic intermediate dihydroxyacetone phosphate, the direct precursor of glycerol-3-phosphate dehydrogenase. The levels of sucrose and various metabolites in the pathway from sucrose to fatty acids remained unaltered. The results show that glycerol-3-phosphate supply co-limits oil accumulation in developing seeds. This has important implications for strategies that aim to increase the overall level of oil in commercial oil-seed crops for use as a renewable alternative to petrol.
Researchers ; Professionals
http://hdl.handle.net/2268/75855
10.1111/j.1467-7652.2007.00252.x

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