Article (Scientific journals)
Increasing seed oil content in oil-seed rape (Brassica napus L.) by over-expression of a yeast glycerol-3-phosphate dehydrogenase under the control of a seed-specific promoter.
Vigeolas, Hélène; Waldeck, Peter; Zank, Thorsten et al.
2007In Plant Biotechnology Journal, 5 (3), p. 431-41
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Keywords :
Brassica napus/embryology/genetics/metabolism; Fatty Acids/metabolism; Glycerolphosphate Dehydrogenase/genetics/metabolism; Glycerophosphates/metabolism; Lipid Metabolism/genetics; Plant Oils/metabolism; Plants, Genetically Modified/metabolism; Promoter Regions, Genetic; Saccharomyces cerevisiae Proteins/genetics/metabolism; Seeds/genetics/metabolism; Transformation, Genetic
Abstract :
[en] Previous attempts to manipulate oil synthesis in plants have mainly concentrated on the genes involved in the biosynthesis and use of fatty acids, neglecting the possible role of glycerol-3-phosphate supply on the rate of triacylglycerol synthesis. In this study, a yeast gene coding for cytosolic glycerol-3-phosphate dehydrogenase (gpd1) was expressed in transgenic oil-seed rape under the control of the seed-specific napin promoter. It was found that a twofold increase in glycerol-3-phosphate dehydrogenase activity led to a three- to fourfold increase in the level of glycerol-3-phosphate in developing seeds, resulting in a 40% increase in the final lipid content of the seed, with the protein content remaining substantially unchanged. This was accompanied by a decrease in the glycolytic intermediate dihydroxyacetone phosphate, the direct precursor of glycerol-3-phosphate dehydrogenase. The levels of sucrose and various metabolites in the pathway from sucrose to fatty acids remained unaltered. The results show that glycerol-3-phosphate supply co-limits oil accumulation in developing seeds. This has important implications for strategies that aim to increase the overall level of oil in commercial oil-seed crops for use as a renewable alternative to petrol.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Vigeolas, Hélène ;  Université de Liège - ULiège > Département des sciences de la vie > Génétique
Waldeck, Peter
Zank, Thorsten
Geigenberger, Peter
Language :
English
Title :
Increasing seed oil content in oil-seed rape (Brassica napus L.) by over-expression of a yeast glycerol-3-phosphate dehydrogenase under the control of a seed-specific promoter.
Publication date :
2007
Journal title :
Plant Biotechnology Journal
ISSN :
1467-7644
eISSN :
1467-7652
Publisher :
Blackwell Publishing, Oxford, United Kingdom
Volume :
5
Issue :
3
Pages :
431-41
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 17 November 2010

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