Reference : Inositol 1,4,5-trisphosphate 3-kinase B controls survival and prevents anergy in B cells
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
Life sciences : Genetics & genetic processes
http://hdl.handle.net/2268/75724
Inositol 1,4,5-trisphosphate 3-kinase B controls survival and prevents anergy in B cells
English
Marechal, Y. [Université Libre de Bruxelles - ULB > Institut de Recherches Interdisciplinaires en Biologie Humaine et Moléculaire (IRIBHM), Institut de Biologie et de Médecine Moléculaires (IBMM), Faculté de Médecine > > >]
Queant, S. [Université Libre de Bruxelles - ULB > Institut de Recherches Interdisciplinaires en Biologie Humaine et Moléculaire (IRIBHM), Institut de Biologie et de Médecine Moléculaires (IBMM), Faculté de Médecine > > >]
Polizzi, S. [Université Libre de Bruxelles - ULB > Institut de Recherches Interdisciplinaires en Biologie Humaine et Moléculaire (IRIBHM), Institut de Biologie et de Médecine Moléculaires (IBMM), Faculté de Médecine > > >]
Pouillon, V. [Université Libre de Bruxelles - ULB > Institut de Recherches Interdisciplinaires en Biologie Humaine et Moléculaire (IRIBHM), Institut de Biologie et de Médecine Moléculaires (IBMM), Faculté de Médecine > > >]
Schurmans, Stéphane mailto [Université de Liège - ULg > Département de sciences fonctionnelles, Biochimie métabolique vétérinaire > et GIGA-Research > > >]
2011
Immunobiology
Gustav Fischer Verlag
216
103-109
Yes (verified by ORBi)
International
0171-2985
Stuttgart
Germany
[en] B cell survival ; Anergy ; Bim ; Itpkb ; Lymphocyte selection
[en] Inositol 1,4,5-trisphosphate 3-kinase B (or Itpkb) and inositol 1,3,4,5-tetrakisphosphate (Ins(1,3,4,5)P4), its reaction product, play an important role in the control of B lymphocyte fate and function in vivo. In order to investigate the fine mechanisms of Itpkb and Ins(1,3,4,5)P4 action in B cells, we crossed Itpkb(-/-) mice with transgenic mice expressing a 3-83mudelta B cell receptor (BCR) specific for membrane-bound MHC-I H2-K(b) and H2-K(k) molecules. On a non-deleting H2-K(d) genetic background, we show that Itpkb is important for the control of Bim protein expression and B cell survival rather than for the control of B cell development from one stage to another. Analyses of cell surface markers expression, proapoptotic Bim protein expression, in vitro survival and in vivo turnover demonstrated that BCR transgenic Itpkb(-/-) B cells exhibit an anergic phenotype with the notable exception of their enhanced antigen-induced calcium signalling. On a deleting H2-K(b) genetic background, we show that Itpkb is not essential for BCR editing or negative selection. These data establish Itpkb as an important regulator of B cell survival and anergy in vivo.
Researchers ; Professionals ; Students
http://hdl.handle.net/2268/75724
10.1016/j.imbio.2010.03.012

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