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Graft of autologous fibroblasts in gingival tissue in vivo after culture in vitro. Preliminary study on rats.
Simain-Sato, Franklin; Lahmouzi, Jamila; Heinen, Ernst et al.
1999In Journal of Periodontal Research, 34 (6), p. 323-8
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Keywords :
Animals; Cell Culture Techniques/methods; Cell Separation/methods; Cells, Cultured; Fibroblasts/cytology/transplantation; Fluorescent Dyes; Gingiva/cytology/transplantation; Male; Microscopy, Fluorescence; Rats; Rats, Wistar; Syringes; Transplantation, Autologous
Abstract :
[en] Several grafting techniques and guided tissue regeneration techniques (GTR) have been well-developed in periodontal surgery. However, these techniques could induce pain and side effects, such as a gingival recession during the healing period following the therapy. The graft of a small autologous connective tissue, using non-invasive surgical techniques could yield several benefits for the patients. Our preliminary study explores the feasibility of collecting healthy gingival tissues, culturing them in vitro to amplify rat gingival fibroblasts (RGF) and inoculating the obtained cells into autologous rat gingival tissues in vivo. Gingival tissues samples were cultured as explants as described by Freshney et al. and Adolphe. Confluent cells surrounding explants were detached after 7 d of culture from Petri dishes using 0.05% trypsin and designated "first transferred cells" (T1). At the third passage (T3), cells cultured as monolayer were either examined under microscopy--phase contrast, scanning, or transmission electron--or numerated after trypan blue exclusion test. Autologous RGF labelled with fluorochrome were inoculated at the vestibular and palatine site of gingival tissue close to the superior incisors. In this preliminary study, 12 Wistar rats were used; for each, 2 biopsies were dissected and fixed for phase contrast or fluorescence microscopy. On d 1, 3 and 7 after injection in rat gingival tissues, fluorochrome-labelled cells could be detected in all these.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Simain-Sato, Franklin ;  Centre Hospitalier Universitaire de Liège - CHU > Médecine dentaire
Lahmouzi, Jamila ;  Centre Hospitalier Universitaire de Liège - CHU > Médecine dentaire
Heinen, Ernst ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Defresne, Marie-Paule ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie - Cytologie
De Pauw-Gillet, Marie-Claire ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie - Cytologie - Département des sciences biomédicales et précliniques
Grisar, Thierry ;  Centre Hospitalier Universitaire de Liège - CHU > Neurologie Sart Tilman
Legros, Jean-Jacques ;  Centre Hospitalier Universitaire de Liège - CHU > Endocrinologie clinique
Legrand, Roman ;  Université de Liège - ULiège > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine)
Language :
English
Title :
Graft of autologous fibroblasts in gingival tissue in vivo after culture in vitro. Preliminary study on rats.
Publication date :
1999
Journal title :
Journal of Periodontal Research
ISSN :
0022-3484
eISSN :
1600-0765
Publisher :
Munksgaard International Publishers, Copenhagen, Denmark
Volume :
34
Issue :
6
Pages :
323-8
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 15 November 2010

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