Reference : Graft of autologous fibroblasts in gingival tissue in vivo after culture in vitro. Preli...
Scientific journals : Article
Life sciences : Anatomy (cytology, histology, embryology...) & physiology
http://hdl.handle.net/2268/75660
Graft of autologous fibroblasts in gingival tissue in vivo after culture in vitro. Preliminary study on rats.
English
Simain-Sato, Franklin mailto [Centre Hospitalier Universitaire de Liège - CHU > > Médecine dentaire >]
Lahmouzi, Jamila mailto [Centre Hospitalier Universitaire de Liège - CHU > > Médecine dentaire >]
Heinen, Ernst mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie humaine >]
Defresne, Marie-Paule mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie - Cytologie >]
De Pauw, Marie-Claire mailto [Université de Liège - ULg > Département des sciences biomédicales et précliniques > Histologie - Cytologie - Département des sciences biomédicales et précliniques >]
Grisar, Thierry mailto [Centre Hospitalier Universitaire de Liège - CHU > > Neurologie Sart Tilman >]
Legros, Jean-Jacques mailto [Centre Hospitalier Universitaire de Liège - CHU > > Endocrinologie clinique >]
Legrand, Roman mailto [Université de Liège - ULg > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine) >]
1999
Journal of Periodontal Research
Munksgaard International Publishers
34
6
323-8
Yes (verified by ORBi)
International
0022-3484
1600-0765
Copenhagen
Denmark
[en] Animals ; Cell Culture Techniques/methods ; Cell Separation/methods ; Cells, Cultured ; Fibroblasts/cytology/transplantation ; Fluorescent Dyes ; Gingiva/cytology/transplantation ; Male ; Microscopy, Fluorescence ; Rats ; Rats, Wistar ; Syringes ; Transplantation, Autologous
[en] Several grafting techniques and guided tissue regeneration techniques (GTR) have been well-developed in periodontal surgery. However, these techniques could induce pain and side effects, such as a gingival recession during the healing period following the therapy. The graft of a small autologous connective tissue, using non-invasive surgical techniques could yield several benefits for the patients. Our preliminary study explores the feasibility of collecting healthy gingival tissues, culturing them in vitro to amplify rat gingival fibroblasts (RGF) and inoculating the obtained cells into autologous rat gingival tissues in vivo. Gingival tissues samples were cultured as explants as described by Freshney et al. and Adolphe. Confluent cells surrounding explants were detached after 7 d of culture from Petri dishes using 0.05% trypsin and designated "first transferred cells" (T1). At the third passage (T3), cells cultured as monolayer were either examined under microscopy--phase contrast, scanning, or transmission electron--or numerated after trypan blue exclusion test. Autologous RGF labelled with fluorochrome were inoculated at the vestibular and palatine site of gingival tissue close to the superior incisors. In this preliminary study, 12 Wistar rats were used; for each, 2 biopsies were dissected and fixed for phase contrast or fluorescence microscopy. On d 1, 3 and 7 after injection in rat gingival tissues, fluorochrome-labelled cells could be detected in all these.
http://hdl.handle.net/2268/75660
also: http://hdl.handle.net/2268/23181

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