[en] Several grafting techniques and guided tissue regeneration techniques (GTR) have been well-developed in periodontal surgery. However, these techniques could induce pain and side effects, such as a gingival recession during the healing period following the therapy. The graft of a small autologous connective tissue, using non-invasive surgical techniques could yield several benefits for the patients. Our preliminary study explores the feasibility of collecting healthy gingival tissues, culturing them in vitro to amplify rat gingival fibroblasts (RGF) and inoculating the obtained cells into autologous rat gingival tissues in vivo. Gingival tissues samples were cultured as explants as described by Freshney et al. and Adolphe. Confluent cells surrounding explants were detached after 7 d of culture from Petri dishes using 0.05% trypsin and designated "first transferred cells" (T1). At the third passage (T3), cells cultured as monolayer were either examined under microscopy--phase contrast, scanning, or transmission electron--or numerated after trypan blue exclusion test. Autologous RGF labelled with fluorochrome were inoculated at the vestibular and palatine site of gingival tissue close to the superior incisors. In this preliminary study, 12 Wistar rats were used; for each, 2 biopsies were dissected and fixed for phase contrast or fluorescence microscopy. On d 1, 3 and 7 after injection in rat gingival tissues, fluorochrome-labelled cells could be detected in all these.
Simain-Sato, Franklin ; Centre Hospitalier Universitaire de Liège - CHU > Médecine dentaire
Lahmouzi, Jamila ; Centre Hospitalier Universitaire de Liège - CHU > Médecine dentaire
Heinen, Ernst ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Defresne, Marie-Paule ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie - Cytologie
De Pauw-Gillet, Marie-Claire ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie - Cytologie - Département des sciences biomédicales et précliniques
Grisar, Thierry ; Centre Hospitalier Universitaire de Liège - CHU > Neurologie Sart Tilman
Legros, Jean-Jacques ; Centre Hospitalier Universitaire de Liège - CHU > Endocrinologie clinique
Legrand, Roman ; Université de Liège - ULiège > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine)
Language :
English
Title :
Graft of autologous fibroblasts in gingival tissue in vivo after culture in vitro. Preliminary study on rats.
Publication date :
1999
Journal title :
Journal of Periodontal Research
ISSN :
0022-3484
eISSN :
1600-0765
Publisher :
Munksgaard International Publishers, Copenhagen, Denmark
Nyman S. New attachment following surgical treatment of human periodontal disease. J Clin Periodontol 1982;9:290-296.
Koichi I. Adjacent gingival recession treated with expanded polytetrafluoroethylene membranes. A report of 2 cases. J Periodontol 1996;67:443-450.
Pini Prato G. Guided tissue regeneration versus mucogingival surgery in the treatment of human buccal recessions. A 4-year follow-up study. J Periodontol 1996;67:1216-1223.
Gotllow J, Nyman S. New attachment formation in the human periodontium by guided tissue regeneration. Case reports. J Clin Periodontol 1986;13:604-616.
Nyman S, Gottlow J. New attachment formation by guided tissue regeneration. J Periodont Res 1987;22:252-254.
Freshney IR. A manual of basic techniques. Cult Animal Cells 1994;104.
Adolphe M. Culture de cellules animales. Methodologies-Applications 1988;2.
Borghetti A. Thick gingival autograft for the coverage of gingival recession. A clinical evaluation. Int J Periodont Rest Dent 1990; 10:217-229.
Edel A, et al. Histologic changes following the grafting of connective tissue into human gingiva. Oral Surg Oral Med Oral Pathol 1977;43:190-195.
Björn H. Coverage of denuded root surfaces with a lateral sliding flap. Use of free gingival grafts. Odontol Rev 1971;22:37-44.
Grupe HE. Warren RF. Repair of gingival defects by a sliding flap operation. J Periodonlol 1956;2:92-95.
Pini Prato G. GTR versus mucogingival surgery in the treatment of human buccal recession. J Periodontol 1992;63:919-928.
Trombelli L. Combined guided tissue regeneration, root conditioning, and fibrin-fibronectin system application in the treatment of gingival recession. A 15-case report. J Periodontol 1994;65:796-803.
Tinti C. Guided tissue regeneration in mucogingival surgery. J Periodontol 1993;64:1184-1191.
Van Dijk LJ. Cell-seeding of periodontal ligament fibroblasts. A novel technique to create new attachment. J Clin Periodontol 1991;18:196-199.
Bokyo GA. formation of new periodontal ligament by periodontal ligament cells implanted in vivo after culture in vitro. J Periodont Res 1981;16:73-88.
Lang HJ. Formation of differentiated tissues in vivo by periodontal cell population cultured in vitro. J Dent Reso 1995;74:1219-1225.
Garrity GJ. Detection of cell culture mycoplasmas by a genetic probe. Exp Cell Res 1986;163:273-278.
Haugland RP. Membrane - permeant reactive tracers for long-term cell labelling. Handbook of fluorescent probes and research chemicals, 6th edn. Molecular Probes 1998;328-329.
Ten Cate AR. The fibroblast and its products. In: Oral histology: developmental structures and function. Toronto: CV Mosby, 1989;90-105.
Fuioka H. Transplantation of dye-labelled hepatocytes. Cell Transplant 1994;3.
Vroemen J. Transplantation of isolated hepatocytes into the pancreas. Eur Surg Res 1988;20:1-11.
Weston SA. New fluorescent dyes for lymphocyte migration studies. Analysis by flow cytometry and fluorescent microscopy. J Immunol Methods 1990;133:87-96.
Paramore CG. Fluorescent labelling of dissociated fetal cells for tissue culture. J Neurosci Methods 1992;44:7-17.
Karrer. Fluorescein labelling of murine hepatocytes for identification after intrahepatic transplantation. Transplantation 1992;24:2820-2821.