[en] The thymic stroma plays a critical role in the generation of T lymphocytes by direct cell-to-cell contacts as well as by secreting growth factors or hormones. The thymic epithelial cells, responsible for thymic hormone secretion, include morphologically and antigenically distinct subpopulations that may exert different roles in thymocyte maturation. The recent development of thymic epithelial cell lines provided an interesting model for studying thymic epithelial influences on T cell differentiation. Treating mouse thymocytes by supernatants from one of TEC line (IT-76M1), we observed an induction of thymocyte proliferation and an increase in the percentages of CD4-/CD8- thymocytes. This proliferation was largely inhibited when thymocytes were incubated with IT-76M1 supernatants together with an anti-thymulin monoclonal antibody, but could be enhanced by pretreating growing epithelial cells by triiodothyronine. We suggest that among the target cells for thymulin within the thymus, some putative precursors of early phenotype might be included.
Le, Tuck, Dinarello, Haynes, Singer J. Immunol 1987, 138:2520.
Cohen-Kaminsky, Delattre, Devergne, Rouet, Gimond, Galanaud, Berrih-Aknin Abstracts of the 10th International Conference of Lymphatic Tissues and Germinal Centres in Immune Reactions , Compiégne, France; 1990, 110.
Denning, Kurtzberg, Le, Tuck, Singer, Haynes Proc. Natl. Acad. Sci. USA 1988, 85:3125.
van de Wijngaert, Kendall, Schuurman, Rademakers, Kater Cell Tiss. Res 1984, 237:227.
Van Vliet, Melis, Van Ewijk Eur. J. Immunol 1984, 14:524.
Savino, Dardenne J. Histochem. Cytochem 1988, 36:1123.
Haynes, Scearce, Lobach, Hensley J. Exp. Med 1984, 159:1149.
Wekerle, Ketelsen Nature 1980, 283:402.
Wekerle, Ketelsen, Ernst J. Exp. Med 1980, 151:925.
Nakayama, Wekerle, Savino, Dardenne, Bach Abstracts of the 6th European Immunology Meeting , Interlaken, Switzerland; 1984, 264.
Defresne (1986) Le microenvironnement thymique et les cellules préleucémiques au cours du developpement des lymphomes induits chez la souris. Role des cellules ‘nurses’ thymiques. Thesis, Liège University, Belgium; .
Imhof, Deugnier, Girault, Champion, Damais, Itoh, Thiery Proc. Natl. Acad. Sci. USA 1988, 85:7699.
Bauvois, Ezine, Imhof, Denoyelle, Thiery J. Immunol 1989, 143:1077.
Itoh, Doi, Kasahara Abstracts of the 6th International Congress on Immunology , Toronto, Canada; 1986, 27.
Cirne-Lima, Savino Abstracts of IV Annual Reunion of Experimental Biology Societies Federation , Caxambu, Brazil; 1989, 421.
Julius, Simpson, Herzenberg Eur. J. Immunol 1973, 3:645.
Dardenne, Pleau, Savino, Bach Immunol. Lett 1982, 4:79.
Savino, Dardenne, Papiernik, Bach (1981) Thymic hormone-containing cells. Characterization and localization of serum thymic factor in young mouse thymus studied by monoclonal antibodies. Journal of Experimental Medicine 156:628.
Berrih, Savino, Azoulay, Dardenne, Bach J. Histochem. Cytochem 1984, 32:432.
Pierres, Naquet, Van Aythoven, Bekkhoucha, Denizot, Mishal, Schimitt-Verhulst, Pierres J. Immunol 1984, 132:2775.
Ledbetter, Herzenberg Immunol. Rev 1979, 47:63.
Malek, Robb, Shevach Proc. Natl. Acad. Sci. USA 1983, 80:5694.
Haskins, Hannum, White, Roehm, Kubo, Kappler, Marrack J. Exp. Med 1984, 160:452.
Lannes-Vieira, van der Meide, Savino J. Immunol. Immunopharmacol , (Abstract); 1989, 9:136.
Hensen, Hoefsmit, Van Den Tweel Clin. Exp. Immunol 1978, 32:309.
Kruisbeek, Zijlstra, Krose J. Immunol 1980, 125:995.