Reference : Collagen fibril disruption occurs early in primary guinea pig knee osteoarthritis.
Scientific journals : Article
Human health sciences : Rheumatology
http://hdl.handle.net/2268/75331
Collagen fibril disruption occurs early in primary guinea pig knee osteoarthritis.
English
Huebner, J. L. [> > > >]
Williams, J. M. [> > > >]
Deberg, Michelle mailto [Université de Liège - ULg > Département des sciences de la motricité > Unité de recherche sur l'os et le cartillage (U.R.O.C.) >]
Henrotin, Yves mailto [Université de Liège - ULg > Département des sciences de la motricité > Unité de recherche sur l'os et le cartillage (U.R.O.C.) - Didactique des sciences de la santé - Pathologie générale et physiopathologie >]
Kraus, V. B. [> > > >]
2010
Osteoarthritis and Cartilage
W.B. Saunders
18
3
397-405
Yes (verified by ORBi)
International
1063-4584
London
United Kingdom
[en] Animals ; Biological Markers/metabolism ; Cartilage, Articular/metabolism ; Collagen Type II/metabolism ; Disease Models, Animal ; Fibril-Associated Collagens/metabolism ; Guinea Pigs ; Immunohistochemistry ; Knee Joint/pathology ; Male ; Osteoarthritis, Knee/pathology ; Time Factors
[en] OBJECTIVE: A major barrier inhibiting the discovery of structural modifying agents for osteoarthritis (OA) is an incomplete understanding of early disease events. Herein, we investigated the time course of collagen II cleavage and fibril disruption in the well-validated Hartley guinea pig model of spontaneous OA of the knee. METHODS: Knee joints of 46 male Hartley guinea pigs were analyzed at 3 weeks, 2, 4, 7, 10, 12, and 18 months of age for histological severity of OA, cartilage collagen fibril disruption by semi-quantitative polarized light microscopy, and expression of type II collagen degradation biomarkers, 9A4 and Coll2-1, by immunohistochemistry. In addition, serum biomarkers specific for collagen II degradation, CTX-II, C2C, and Coll2-1 were quantified. RESULTS: Collagen fibril disruption and expression of the collagenase-generated cleavage neoepitope, 9A4, were observed as early as 2 months of age, despite the appearance of histological OA at 4 months of age. Only serum Coll2-1 increased coincident with the early disruption of the collagen fibril between 3 weeks and 7 months, in contrast to serum C2C, which did not change significantly or correlate with histological severity. Inversely, CTX-II declined dramatically from 3 weeks to 4 months and remaining low thereafter, coincident with growth plate turnover. CONCLUSIONS: Collagenase cleavage and disruption of the type II collagen network are early OA disease events in this model, preceding histological evidence of proteoglycan loss. The markedly different serum profiles of collagen II-related biomarkers during the early stages of disease development suggest compartmental segregation and temporal regulation of collagen degrading enzymes.
http://hdl.handle.net/2268/75331
10.1016/j.joca.2009.09.011
Copyright 2009 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

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