Reference : Optimisation and validation of a fast HPLC method for the quantification of sulindac ...
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
http://hdl.handle.net/2268/75222
Optimisation and validation of a fast HPLC method for the quantification of sulindac and its related impurities
English
Krier, Fabrice mailto [Université de Liège - ULg > Département de pharmacie > Pharmacie galénique >]
Brion, Michaël [> >]
Debrus, Benjamin mailto [Université de Liège - ULg > Département de pharmacie > Chimie analytique >]
Lebrun, Pierre mailto [Université de Liège - ULg > Département de pharmacie > Chimie analytique >]
Driesen, Aurélie [> >]
Ziemons, Eric mailto [Université de Liège - ULg > Département de pharmacie > Chimie analytique >]
Evrard, Brigitte mailto [Université de Liège - ULg > Département de pharmacie > Pharmacie galénique >]
Hubert, Philippe mailto [Université de Liège - ULg > Département de pharmacie > Chimie analytique >]
2011
Journal of Pharmaceutical & Biomedical Analysis
Elsevier Science
54
694-700
Yes (verified by ORBi)
International
0731-7085
Oxford
United Kingdom
[en] HPLC ; Sulindac ; Validation ; Design space ; Optimisation ; sub-2micron column
[en] The European Pharmacopoeia describes a liquid chromatography (LC) method for the quantification of sulindac, using a quaternary mobile phase including chloroform and with a
rather long run time. In the present study, a new method using a short sub-2μm column, which can be used on a classical HPLC system, was developed. The new LC conditions (without chloroform) were optimised by means of a new methodology based on design of experiments in order to obtain an optimal separation. Four factors were studied: the duration of the initial isocratic step, the percentage of organic modifier at the beginning of the gradient, the percentage of organic modifier at the end of the gradient and the gradient time. The optimal condition allows the separation of sulindac and of its 3 related impurities in six minutes instead of 18 min. Finally, the method was successfully validated using an accuracy profile approach in order to demonstrate its ability to accurately quantify these compounds.
C.I.R.M.
Région wallonne : Direction générale des Technologies, de la Recherche et de l'Energie - DGTRE
Researchers ; Professionals
http://hdl.handle.net/2268/75222

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